Effects of hydroxylpropyl-β-cyclodextrin on in vitro insulin stability

Int J Mol Sci. 2009 May 6;10(5):2031-2040. doi: 10.3390/ijms10052031.

Abstract

The objective of this study was to elucidate the effects of hydroxylpropyl-beta-cyclodextrin (HP-beta-CD) on the in vitro stability of insulin. It was found that HP-beta-CD had positive effects on the stability of insulin in acid and base and under high temperature conditions. Furthermore, use of HP-beta-CD could also increase the stability of disulfide bonds which are important to the conformation of insulin. Through (1)H-NMR experiments it was found that the protective effect of HP-beta-CD was due to complexation with insulin. The results suggest that the presence of HP-beta-CD could improve the stability of insulin in different environments.

Keywords: Protection; cyclodextrin; insulin; stability.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Hydroxypropyl-beta-cyclodextrin
  • Cyclodextrins / chemistry
  • Cyclodextrins / metabolism*
  • Drug Stability
  • Hot Temperature
  • Hydrogen-Ion Concentration
  • Insulin / metabolism*
  • Pharmaceutical Preparations / chemistry
  • Pharmaceutical Preparations / metabolism*
  • beta-Cyclodextrins / chemistry
  • beta-Cyclodextrins / metabolism*

Substances

  • Cyclodextrins
  • Insulin
  • Pharmaceutical Preparations
  • beta-Cyclodextrins
  • 2-Hydroxypropyl-beta-cyclodextrin