Magnetic Properties of Bacterial Magnetosomes Produced by Magnetospirillum caucaseum SO-1

Kamil G Gareev; Denis S Grouzdev; Peter V Kharitonskii; Demid A Kirilenko; Andrei Kosterov; Veronika V Koziaeva; Vladimir S Levitskii; Gabriele Multhoff; Elina K Nepomnyashchaya; Andrey V Nikitin; Anastasia Nikitina; Elena S Sergienko; Stanislav M Sukharzhevskii; Evgeniy I Terukov; Valentina V Trushlyakova; Maxim Shevtsov

doi:10.3390/microorganisms9091854

Magnetic Properties of Bacterial Magnetosomes Produced by Magnetospirillum caucaseum SO-1

Microorganisms. 2021 Aug 31;9(9):1854. doi: 10.3390/microorganisms9091854.

Authors

Kamil G Gareev¹, Denis S Grouzdev², Peter V Kharitonskii³, Demid A Kirilenko⁴, Andrei Kosterov⁵, Veronika V Koziaeva⁶, Vladimir S Levitskii⁷, Gabriele Multhoff⁸, Elina K Nepomnyashchaya⁹, Andrey V Nikitin¹, Anastasia Nikitina^{3

10}, Elena S Sergienko⁵, Stanislav M Sukharzhevskii¹⁰, Evgeniy I Terukov^{1

4

7}, Valentina V Trushlyakova¹, Maxim Shevtsov^{8

11

12}

Affiliations

¹ Department of Micro and Nanoelectronics, Saint Petersburg Electrotechnical University "LETI", 197376 Saint Petersburg, Russia.
² SciBear OU, Tartu mnt 67/1-13b, Kesklinna Linnaosa, 10115 Tallinn, Estonia.
³ Department of Physics, Saint Petersburg Electrotechnical University "LETI", 197376 Saint Petersburg, Russia.
⁴ Centre of Nanoheterostructure Physics, Ioffe Institute, 194021 Saint Petersburg, Russia.
⁵ Department of Earth Physics, Saint Petersburg University, 199034 Saint Petersburg, Russia.
⁶ Research Center of Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, 119071 Moscow, Russia.
⁷ R&D Center TFTE LLC, 194021 Saint Petersburg, Russia.
⁸ Center of Translational Cancer Research (TranslaTUM), Klinikum Rechts der Isar, Technical University Munich, 81675 Munich, Germany.
⁹ Institute of Electronics and Telecommunications, Peter the Great St. Petersburg Polytechnic University, 195251 Saint Petersburg, Russia.
¹⁰ Magnetic Resonance Research Centre, Saint Petersburg University, 199034 Saint Petersburg, Russia.
¹¹ Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences, 194064 Saint Petersburg, Russia.
¹² Personalized Medicine Centre, Almazov National Medical Research Centre, 197341 Saint Petersburg, Russia.

Abstract

In this study, the magnetic properties of magnetosomes isolated from lyophilized magnetotactic bacteria Magnetospirillum caucaseum SO-1 were assessed for the first time. The shape and size of magnetosomes and cell fragments were studied by electron microscopy and dynamic light scattering techniques. Phase and elemental composition were analyzed by X-ray and electron diffraction and Raman spectroscopy. Magnetic properties were studied using vibrating sample magnetometry and electron paramagnetic resonance spectroscopy. Theoretical analysis of the magnetic properties was carried out using the model of clusters of magnetostatically interacting two-phase particles and a modified method of moments for a system of dipole-dipole-interacting uniaxial particles. Magnetic properties were controlled mostly by random aggregates of magnetosomes, with a minor contribution from preserved magnetosome chains. Results confirmed the high chemical stability and homogeneity of bacterial magnetosomes in comparison to synthetic iron oxide magnetic nanoparticles.

Keywords: Magnetospirillum caucaseum SO-1; bacterial magnetosomes; magnetic properties; magnetostatic interaction; magnetotactic bacteria; theoretical modeling.

Abstract

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