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Page 1
Showing results for single guv
Search for Songle Guo instead (1 results)
A Single GUV Method for Revealing the Action of Cell-Penetrating Peptides in Biomembranes.
Moghal MMR, Shuma ML, Islam MZ, Yamazaki M. Moghal MMR, et al. Methods Mol Biol. 2022;2383:167-179. doi: 10.1007/978-1-0716-1752-6_11. Methods Mol Biol. 2022. PMID: 34766289
The mechanism of entry of cell-penetrating peptides (CPPs) into the cytosol of various cells has been studied by examining the interaction of CPPs with lipid bilayers and their entry into lipid vesicle lumens using various methods. Here we describe a single giant unilamell …
The mechanism of entry of cell-penetrating peptides (CPPs) into the cytosol of various cells has been studied by examining the interaction o …
Action of antimicrobial peptides and cell-penetrating peptides on membrane potential revealed by the single GUV method.
Moghal MMR, Hossain F, Yamazaki M. Moghal MMR, et al. Biophys Rev. 2020 Apr;12(2):339-348. doi: 10.1007/s12551-020-00662-z. Epub 2020 Mar 9. Biophys Rev. 2020. PMID: 32152921 Free PMC article. Review.
In this short review, we summarize the results of the effect of membrane potential on the action of an AMP, lactoferricin B (LfcinB), and a CPP, transportan 10 (TP10), in vesicle membranes revealed by the single giant unilamellar vesicle (GUV) method. Parts of the a …
In this short review, we summarize the results of the effect of membrane potential on the action of an AMP, lactoferricin B (LfcinB), and a …
Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers.
Wang Y, Kumar A, Jin H, Zhang Y. Wang Y, et al. Biophys J. 2021 Dec 21;120(24):5454-5465. doi: 10.1016/j.bpj.2021.11.2884. Epub 2021 Nov 20. Biophys J. 2021. PMID: 34813728 Free PMC article.
The trapped GUVs with high membrane tension can serve as a force sensor to accurately detect reversible folding of a DNA hairpin or membrane binding of synaptotagmin-1 C2AB domain attached to the GUV. We also observed that SUVs are rigid enough to resist large pulling forc …
The trapped GUVs with high membrane tension can serve as a force sensor to accurately detect reversible folding of a DNA hairpin or membrane …
The single GUV method for revealing the functions of antimicrobial, pore-forming toxin, and cell-penetrating peptides or proteins.
Islam MZ, Alam JM, Tamba Y, Karal MA, Yamazaki M. Islam MZ, et al. Phys Chem Chem Phys. 2014 Aug 14;16(30):15752-67. doi: 10.1039/c4cp00717d. Phys Chem Chem Phys. 2014. PMID: 24965206
We compare the single GUV method with other methods for investigating the interaction of peptides/proteins with lipid membranes (i.e., the large unilamellar vesicle (LUV) suspension method, the GUV suspension method, and single channel recording), and …
We compare the single GUV method with other methods for investigating the interaction of peptides/proteins with lipid membrane …
Single GUV method reveals interaction of tea catechin (-)-epigallocatechin gallate with lipid membranes.
Tamba Y, Ohba S, Kubota M, Yoshioka H, Yoshioka H, Yamazaki M. Tamba Y, et al. Biophys J. 2007 May 1;92(9):3178-94. doi: 10.1529/biophysj.106.097105. Epub 2007 Feb 9. Biophys J. 2007. PMID: 17293394 Free PMC article.
In this study, we investigated the interaction of (-)-epigallocatechin gallate (EGCg), a major catechin in tea extract, with single giant unilamellar vesicles (GUVs) of egg phosphatidylcholine (egg PC) using phase-contrast fluorescence microscopy and the single G …
In this study, we investigated the interaction of (-)-epigallocatechin gallate (EGCg), a major catechin in tea extract, with single g …
Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture.
Bashirzadeh Y, Redford SA, Lorpaiboon C, Groaz A, Moghimianavval H, Litschel T, Schwille P, Hocky GM, Dinner AR, Liu AP. Bashirzadeh Y, et al. Commun Biol. 2021 Sep 28;4(1):1136. doi: 10.1038/s42003-021-02653-6. Commun Biol. 2021. PMID: 34584211 Free PMC article.
In vitro experiments and coarse-grained simulations have shown that the actin crosslinking proteins alpha-actinin and fascin segregate into distinct domains in single actin bundles with a molecular size-dependent competition-based mechanism. Here, by encapsulating actin, a …
In vitro experiments and coarse-grained simulations have shown that the actin crosslinking proteins alpha-actinin and fascin segregate into …
Elementary Processes and Mechanisms of Interactions of Antimicrobial Peptides with Membranes-Single Giant Unilamellar Vesicle Studies.
Hasan M, Yamazaki M. Hasan M, et al. Adv Exp Med Biol. 2019;1117:17-32. doi: 10.1007/978-981-13-3588-4_3. Adv Exp Med Biol. 2019. PMID: 30980351 Review.
In this mini-review, we summarize the present state of knowledge of the interactions of AMPs with lipid vesicles obtained using the single giant unilamellar vesicle (GUV) method. Currently, three modes of action of AMPs on GUVs have been defined. The elementary proc …
In this mini-review, we summarize the present state of knowledge of the interactions of AMPs with lipid vesicles obtained using the singl …
The role of membrane tension in the action of antimicrobial peptides and cell-penetrating peptides in biomembranes.
Hasan M, Moghal MMR, Saha SK, Yamazaki M. Hasan M, et al. Biophys Rev. 2019 Jun;11(3):431-448. doi: 10.1007/s12551-019-00542-1. Epub 2019 May 15. Biophys Rev. 2019. PMID: 31093936 Free PMC article. Review.
However, the elementary processes and mechanisms of their functions have not been clear. The single giant unilamellar vesicle (GUV) method has revealed the details of elementary processes of interaction of some AMPs and CPPs with lipid vesicles. ...First, we explain …
However, the elementary processes and mechanisms of their functions have not been clear. The single giant unilamellar vesicle (GUV …
Fast 3D imaging of giant unilamellar vesicles using reflected light-sheet microscopy with single molecule sensitivity.
Jannasch A, Szilagyi SA, Burmeister M, Davis QT, Hermsdorf GL, De S, Schäffer E. Jannasch A, et al. J Microsc. 2022 Jan;285(1):40-51. doi: 10.1111/jmi.13070. Epub 2021 Dec 8. J Microsc. 2022. PMID: 34786705
Observation of highly dynamic processes inside living cells at the single molecule level is key for a better understanding of biological systems. ...We demonstrate fast volume scanning by imaging a two-colour giant unilamellar vesicle (GUV) hemisphere. In addition, …
Observation of highly dynamic processes inside living cells at the single molecule level is key for a better understanding of biologi …
Micron-sized domains in quasi single-component giant vesicles.
Knorr RL, Steinkühler J, Dimova R. Knorr RL, et al. Biochim Biophys Acta Biomembr. 2018 Oct;1860(10):1957-1964. doi: 10.1016/j.bbamem.2018.06.015. Epub 2018 Jun 30. Biochim Biophys Acta Biomembr. 2018. PMID: 29963995 Free article.
In doing so, many research teams rely on standard protocols for GUV preparation and handling involving the use of sugar solutions. ...In addition, upon further cooling of the GUVs down to the immediate vicinity of T(M), stripe-like dye-enriched structures around the domain …
In doing so, many research teams rely on standard protocols for GUV preparation and handling involving the use of sugar solutions. .. …
117 results