Comparative Degradation of a Thiazole Pollutant by an Advanced Oxidation Process and an Enzymatic Approach

Biomolecules. 2017 Aug 24;7(3):64. doi: 10.3390/biom7030064.

Abstract

Organic pollutants, especially those found in water bodies, pose a direct threat to various aquatic organisms as well as humans. A variety of different remediation approaches, including chemical and biological methods, have been developed for the degradation of various organic pollutants. However, comparative mechanistic studies of pollutant degradation by these different systems are almost non-existent. In this study, the degradation of a model thiazole pollutant, thioflavin T (ThT), was carried out in the presence of either an advanced oxidation process (ultraviolet (UV) + H₂O₂) or a chloroperoxidase enzyme system (CPO + H₂O₂). The degradation was followed both spectrophotometrically and using liquid chromatography-mass spectroscopy (LC-MS), and the products formed were identified using tandem liquid chromatography-mass spectrometry-mass spectrometry (LC-MS-MS). The results show that the two remediation approaches produced different sets of intermediates, with only one common species (a demethylated form of ThT). This suggests that different degradation schemes were operating in the two systems. Interestingly, one of the major intermediates produced by the CPO + H₂O₂ system was a chlorinated form of thioflavin. Phytotoxicity studies showed that the CPO + H₂O₂-treated ThT solution was significantly (p <0.05) less toxic than the UV + H₂O₂-treated ThT solution. This is the first time that a comparative mechanistic study showing in detail the intermediates generated in chemical and biological remediation methods has been presented. Furthermore, the results show that different remediation systems have very different degradation schemes and result in products having different toxicities.

Keywords: advanced oxidation process; bioremediation; chloroperoxidase; enzymes; peroxidases; thiazole.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chloride Peroxidase / metabolism*
  • Chromatography, Liquid
  • Environmental Restoration and Remediation
  • Hydrogen Peroxide / pharmacology
  • Kinetics
  • Oxidation-Reduction
  • Tandem Mass Spectrometry
  • Thiazoles / chemistry*
  • Ultraviolet Rays
  • Water Pollutants, Chemical / chemistry*

Substances

  • Thiazoles
  • Water Pollutants, Chemical
  • Hydrogen Peroxide
  • Chloride Peroxidase