Copaiba Oil-Based Emulsion as a Natural Chemotherapeutic Agent for the Treatment of Bovine Mastitis: In Vivo Studies

Katieli da Silva Souza Campanholi; Ranulfo Combuca da Silva Junior; Flávia Amanda Pedroso de Morais; Renato Sonchini Gonçalves; Bruna Moura Rodrigues; Magali Soares Dos Santos Pozza; Lidiane Vizioli de Castro-Hoshino; Silvio Mayke Leite; Otávio Augusto Capeloto; Mauro Luciano Baesso; Paulo Cesar Pozza; Wilker Caetano

doi:10.3390/pharmaceutics15020346

Copaiba Oil-Based Emulsion as a Natural Chemotherapeutic Agent for the Treatment of Bovine Mastitis: In Vivo Studies

Pharmaceutics. 2023 Jan 20;15(2):346. doi: 10.3390/pharmaceutics15020346.

Affiliations

¹ Chemistry Department, State University of Maringá, Maringá 87020-900, Brazil.
² Centre de Recherches sur les Macromolecules Végétales (CERMAV)-CNRS, Université Grenoble Alpes, 601, rue de la Chimie, BP 53, CEDEX 9, 38041 Grenoble, France.
³ Laboratory of Chemistry of Natural Products, Department of Chemistry, Center for Exact Sciences and Technology, Federal University of Maranhão, São Luís 65080-805, Brazil.
⁴ Animal Science Department, State University of Maringá, Maringá 87020-900, Brazil.
⁵ Physics Department, State University of Maringá, Maringá 87020-900, Brazil.
⁶ Institute of Health and Biotechnology of Coari, Federal University of Amazonas, Coari 69460-000, Brazil.

Abstract

Copaiba oil-resin (COR) extracted from Copaifera reticulata Ducke has been used as a natural chemotherapeutic agent for a wide range of therapeutic applications. This study presents an emulgel design with a high concentration of COR, designed to prevent and treat mastitis. The COR was stabilized in a gel matrix constituted by carbopol C934P and Pluronic^® F127 (ECO formulation) ratios. The permeation study of ECO was accessed by Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS). The results reveal a high capacity of ECO to permeate deep skin layers. Dairy cows with a history of mastitis were used as in vivo models and exposed to ECO treatment. Monitoring of the teat's inflammatory response showed that ECO effectively prevents mastitis. Furthermore, the ECO formulation was able to form a thin film gel on the application side, preventing fly proliferation and significantly reducing the pathogen load. This study reveals a drug that can used as an alternative application for mastitis in human or veterinary clinics.

Keywords: bovine mastitis; copaiba oil-resin; emulgel; natural anti-inflammatory; permeation study; photoacoustic.

Grants and funding

This study was sponsored by the National Ministry of Health (Secretariat of Science, Technology and Strategic Inputs—SCTIE, Department of Science and Technology—DECIT); and Brazilian funding agencies, such as Araucária Foundation/PR and CNPq (National Council for Scientific and Technological Development), through contracts no. 405967/2018-7 and 307695/2020-4, under CAPES (Coordination for the Improvement of Higher Education Personnel) process no. 88887.286821/2018-00 and FINEP (Financier of Studies and Projects).