Cytotoxicity of Triterpenoid Alkaloids from Buxus microphylla against Human Tumor Cell Lines

Shi-Tou Bai; Guo-Lei Zhu; Xing-Rong Peng; Jin-Run Dong; Mu-Yuan Yu; Jian-Chao Chen; Luo-Sheng Wan; Ming-Hua Qiu

doi:10.3390/molecules21091125

Cytotoxicity of Triterpenoid Alkaloids from Buxus microphylla against Human Tumor Cell Lines

Molecules. 2016 Aug 26;21(9):1125. doi: 10.3390/molecules21091125.

Authors

Shi-Tou Bai^{1

2}, Guo-Lei Zhu³, Xing-Rong Peng^{4

5}, Jin-Run Dong^{6

7}, Mu-Yuan Yu⁸, Jian-Chao Chen^{9

10

11}, Luo-Sheng Wan^{12

13}, Ming-Hua Qiu^{14

15

16}

Affiliations

¹ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. baisitou@126.com.
² School of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. baisitou@126.com.
³ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. zhuguolei@mail.kib.ac.cn.
⁴ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. pengxingrong@mail.kib.ac.cn.
⁵ Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. pengxingrong@mail.kib.ac.cn.
⁶ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. dongjinrun@mail.kib.ac.cn.
⁷ Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. dongjinrun@mail.kib.ac.cn.
⁸ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. yumuyuan@mail.kib.ac.cn.
⁹ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. jcchen@mail.kib.ac.cn.
¹⁰ School of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. jcchen@mail.kib.ac.cn.
¹¹ Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. jcchen@mail.kib.ac.cn.
¹² State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. wanlesheng1@163.com.
¹³ Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. wanlesheng1@163.com.
¹⁴ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. mhchiu@mail.kib.ac.cn.
¹⁵ School of Traditional Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China. mhchiu@mail.kib.ac.cn.
¹⁶ Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. mhchiu@mail.kib.ac.cn.

Abstract

Three new triterpenoid alkaloids, namely buxmicrophyllines P-R (1-3), were isolated from the twigs and leaves of Buxus microphylla. Their structures were elucidated on the basis of NMR and MS spectroscopic analyses. Structurally, compounds 1-3 belong to the 9,10-cycloartane type alkaloids. In addition, compound 3 exhibited moderate cytotoxic activities in vitro against HL-60, SMMC-7221, A-549, MCF-7, and SW480 cell lines (with IC50 values ranging from 4.51 to 15.58 μM).

Keywords: Buxus microphylla; cytotoxicity; triterpenoid alkaloid.

MeSH terms

A549 Cells
Alkaloids* / chemistry
Alkaloids* / isolation & purification
Alkaloids* / pharmacology
Antineoplastic Agents, Phytogenic* / chemistry
Antineoplastic Agents, Phytogenic* / isolation & purification
Antineoplastic Agents, Phytogenic* / pharmacology
Buxus / chemistry*
Cytotoxins* / chemistry
Cytotoxins* / isolation & purification
Cytotoxins* / pharmacology
HL-60 Cells
Humans
MCF-7 Cells
Triterpenes* / chemistry
Triterpenes* / isolation & purification
Triterpenes* / pharmacology

Substances

Alkaloids
Antineoplastic Agents, Phytogenic
Cytotoxins
Triterpenes