[Effect of sodium butyrate on apoptosis and stromal interaction molecule and Orai1 activity in human colon cancer HCT-116 cells in vitro]

Nan Fang Yi Ke Da Xue Xue Bao. 2012 Feb;32(2):189-92.
[Article in Chinese]

Abstract

Objective: To investigate the mechanism underlying sodium butyrate (NaB)-induced apoptosis of a human colon cancer cell line HCT-116.

Methods: The apoptosis of HCT-116 cells induced by NaB was confirmed by hoechst33342 staining and AnnexinV+ PI assay. The changes in the intracellular localization of stromal interaction molecule (STIM1) and Orai1 following NaB treatment were detected by immunofluorescence technique. Western blotting was used to investigate the protein expression levels of STIM1 and Orai1.

Results: NaB induced apoptosis and caused translocation and colocalization of STIM1 and Orai1 in HCT-116 cells.

Conclusion: The apoptosis of human colon cancer cells induced by NaB is correlated to the redistribution of STIM1 and Orai1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Butyrates / pharmacology*
  • Calcium Channels / metabolism*
  • HCT116 Cells
  • Histone Deacetylase Inhibitors / pharmacology
  • Humans
  • Membrane Proteins / metabolism*
  • Neoplasm Proteins / metabolism*
  • ORAI1 Protein
  • Stromal Interaction Molecule 1

Substances

  • Antineoplastic Agents
  • Butyrates
  • Calcium Channels
  • Histone Deacetylase Inhibitors
  • Membrane Proteins
  • Neoplasm Proteins
  • ORAI1 Protein
  • ORAI1 protein, human
  • STIM1 protein, human
  • Stromal Interaction Molecule 1