Comparison of Immune-Related Gene Expression in Two Chicken Breeds Following Infectious Bronchitis Virus Vaccination

Schwann Chuwatthanakhajorn; Chi-Sheng Chang; Kannan Ganapathy; Pin-Chi Tang; Chih-Feng Chen

doi:10.3390/ani13101642

Comparison of Immune-Related Gene Expression in Two Chicken Breeds Following Infectious Bronchitis Virus Vaccination

Animals (Basel). 2023 May 15;13(10):1642. doi: 10.3390/ani13101642.

Authors

Schwann Chuwatthanakhajorn^{1

2}, Chi-Sheng Chang³, Kannan Ganapathy⁴, Pin-Chi Tang^{1

5}, Chih-Feng Chen^{1

5

6}

Affiliations

¹ Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan.
² Faculty of Veterinary Science, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
³ Department of Animal Science, Chinese Culture University, Taipei 111, Taiwan.
⁴ Institute of Infection, Veterinary & Ecological Sciences (IVES), University of Liverpool, Neston CH64 7TE, UK.
⁵ The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
⁶ Smart Sustainable New Agriculture Research Center (SMARTer), Taichung 402, Taiwan.

Abstract

This study aims to identify the immune-related genes and the corresponding biological pathways following infectious bronchitis virus vaccination in Taiwan Country and White Leghorn chicken breeds. Transcriptomic analyses of the spleen of these two breeds were conducted by next-generation sequencing. Compared to White Leghorn chicken, Taiwan Country chicken showed a significantly higher level of anti-infectious bronchitis virus (IBV) antibodies at 14 and 21 days pos vaccination. At 7 days post vaccination, in the Taiwan Country chicken, higher expression of mitogen-activated protein kinase 10, Major histocompatibility complex class 1, and V-set pre-B cell surrogate light chain 3 were found. In contrast, the White Leghorn chicken had a high expression of interleukin 4 induced 1, interleukin 6, and interleukin 22 receptor subunit alpha 2. These findings have highlighted the variations in immune induction between chickens with distinct genetic background and provided biological pathways and specific genes involved in immune responses against live attenuated IBV vaccine.

Keywords: RNA-seq; differentially expressed gene; immune response traits; infectious bronchitis virus vaccination; spleen transcriptomic.

Grants and funding

This research were funded by the Ministry of Science and Technology (MOST), Taiwan, grant number MOST 109-2321-B-005-019- and the Smart Sustainable New Agricultural Research Center (SMARTer), grant number NSTC 111-2634-F-005-001-.