Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of Nε-carboxymethyl lysine in food samples

Armando Gómez-Ojeda; Sarahi Jaramillo-Ortíz; Katarzyna Wrobel; Kazimierz Wrobel; Gloria Barbosa-Sabanero; Claudia Luevano-Contreras; Maria Pia de la Maza; Jaime Uribarri; Ma Dolores Del Castillo; Ma Eugenia Garay-Sevilla

doi:10.1016/j.foodchem.2017.09.098

Comparative evaluation of three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of N^ε-carboxymethyl lysine in food samples

Food Chem. 2018 Mar 15:243:11-18. doi: 10.1016/j.foodchem.2017.09.098. Epub 2017 Sep 20.

Affiliations

¹ Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: armando_gomezojeda@hotmail.com.
² Department of Chemistry, University of Guanajuato, Lascurain de Retana # 6, Centro Guanajuato, Gto., Guanajuato CP. 36000, Mexico. Electronic address: jaosarahi@gmail.com.
³ Department of Chemistry, University of Guanajuato, Lascurain de Retana # 6, Centro Guanajuato, Gto., Guanajuato CP. 36000, Mexico. Electronic address: katarzyn@ugto.mx.
⁴ Department of Chemistry, University of Guanajuato, Lascurain de Retana # 6, Centro Guanajuato, Gto., Guanajuato CP. 36000, Mexico. Electronic address: kazimier@ugto.mx.
⁵ Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: gloriabs70@hotmail.com.
⁶ Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: claudialc11@gmail.com.
⁷ University of Chile, Av. Libertador Bernardo O'Higgins 1058, Santiago, Chile. Electronic address: mariapiadelamaza@gmail.com.
⁸ Department of Medicine, The Icahn School of Medicine at Mount Sinai, New York, NY, USA. Electronic address: jaime.uribarri@mountsinai.org.
⁹ Food Bioscience Group, Department of Food Analysis and Bioactivity, Institute of Food Science, Research (CIAL), Spanish National Research Council (CSIC), Nicolas Cabrera 9, Madrid 28049, Spain. Electronic address: mdolores.delcastillo@csic.es.
¹⁰ Department of Medical Science, University of Guanajuato, 20 de enero 929 Col. Obregón Leon, Guanajuato CP. 37320, Mexico. Electronic address: marugaray_2000@yahoo.com.

PMID: 29146316
DOI: 10.1016/j.foodchem.2017.09.098

Abstract

N^ε-carboxymethyl-lysine (CML) is measured in food, but there is a controversy concerning the most convenient yet reliable method(s) for this task. This work compares three different ELISA assays and HPLC-ESI-ITMS/MS for the analysis of CML in several food items. The four methods showed the same decreasing order of CML concentration: beef, bacon>chicken > fish>dairy products>grain products>fruits/vegetables. HPLC-ESI-ITMS/MS results highly correlated with those obtained by ELISA performed with monoclonal CML-antibody (β=0.98, p<0.0001) whereas My Bio Source® kit results were not correlated with those provided by Lamider®. Small differences of CML concentrations in food items prepared by different culinary treatment were clearly distinguished by HPLC-ESI-ITMS/MS, but could not always be detected by ELISA. This work demonstrates a reasonable relationship between CM determined by ELISA and HPLC-ESI-ITMS/MS and therefore supports the implementation of ELISA in food CML/AGEs screening.

Keywords: ELISA; Food; Liquid chromatography – mass spectrometry; N(ε)-carboxymethyl lysine.

Publication types

Comparative Study

MeSH terms

Animals
Cattle
Chromatography, High Pressure Liquid / methods*
Enzyme-Linked Immunosorbent Assay / methods*
Fishes
Food Contamination / analysis
Fruit / chemistry*
Glycation End Products, Advanced / analysis*
Lysine / analogs & derivatives*
Lysine / analysis
Mass Spectrometry / methods*
Meat / analysis*
Swine
Vegetables / chemistry*

Substances

Glycation End Products, Advanced
N(6)-carboxymethyllysine
Lysine