Reference interval of all haematological and biological analytes should be measured for every population because of the huge diversity in genetic make-up, dietary habits. The coverage interval of antioxidant vitamins (vitamin C and α -tocopherol) in the plasma and serum of reference Bengali population was determined and compared with the reference intervals of antioxidant vitamins in the established literature. Adult healthy volunteers from 18 to 68 years of age underwent extensive clinical and investigational procedure and were included in the study. Vitamin C and α -tocopherol were estimated using simple Spectrophotometric method. Of the 71 healthy Bengali volunteers participated, 31 were males and 40 were females. The mean concentration of plasma vitamin C was found to be 0.65 mg/dL. The mean α -tocopherol was found 6.35 mg/L (14.74 μ mol/L) in the study population higher than the normal threshold value for α -tocopherol but lower than other populations. The study data enabled us to determine the gender nonspecific coverage interval of antioxidant vitamins, and the intervals were lower than the established reference interval in other populations.