Characterization of flavonoids and naphthopyranones in methanol extracts of Paepalanthus chiquitensis Herzog by HPLC-ESI-IT-MSn and their mutagenic activity

Fabiana Volpe Zanutto; Paula Karina Boldrin; Eliana Aparecida Varanda; Samara Fernandes de Souza; Paulo Takeo Sano; Wagner Vilegas; Lourdes Campaner dos Santos

doi:10.3390/molecules18010244

Characterization of flavonoids and naphthopyranones in methanol extracts of Paepalanthus chiquitensis Herzog by HPLC-ESI-IT-MSn and their mutagenic activity

Molecules. 2012 Dec 27;18(1):244-62. doi: 10.3390/molecules18010244.

Authors

Fabiana Volpe Zanutto¹, Paula Karina Boldrin, Eliana Aparecida Varanda, Samara Fernandes de Souza, Paulo Takeo Sano, Wagner Vilegas, Lourdes Campaner dos Santos

Affiliation

¹ Department of Organic Chemistry, Institute of Chemistry, UNESP-Sao Paulo State University, Araraquara CEP 14800-900, Sao Paulo, Brazil.

Abstract

A HPLC-ESI-IT-MSn method, based on high-performance liquid chromatography coupled to electrospray negative ionization multistage ion trap mass spectrometry, was developed for rapid identification of 24 flavonoid and naphthopyranone compounds. The methanol extracts of the capitulae and scapes of P. chiquitensis exhibited mutagenic activity in the Salmonella/microsome assay, against strain TA97a.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Eriocaulaceae / chemistry*
Flavonoids / chemistry*
Mutagens / toxicity
Plant Extracts / chemistry*
Plant Extracts / pharmacology
Salmonella / drug effects
Salmonella / growth & development
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry

Substances

Flavonoids
Mutagens
Plant Extracts