Prevention of osteoporosis by oral administration of phytate-removed and deamidated soybean β-conglycinin

Makoto Akao; Ryusuke Abe; Noriko Sato; Atsuko Hasegawa-Tanigome; Hitoshi Kumagai; Hitomi Kumagai

doi:10.3390/ijms16012117

Prevention of osteoporosis by oral administration of phytate-removed and deamidated soybean β-conglycinin

Int J Mol Sci. 2015 Jan 19;16(1):2117-29. doi: 10.3390/ijms16012117.

Authors

Makoto Akao¹, Ryusuke Abe², Noriko Sato³, Atsuko Hasegawa-Tanigome⁴, Hitoshi Kumagai⁵, Hitomi Kumagai⁶

Affiliations

¹ Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. makao@brs.nihon-u.ac.jp.
² Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. r.abe0615@gmail.com.
³ Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. foodchem@brs.nihon-u.ac.jp.
⁴ Department of Food Business, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. tanigome.atsuko@nihon-u.ac.jp.
⁵ Department of Food Science and Nutrition, Kyoritsu Women's University, 2-2-1 Hitotsubashi, Chiyoda-ku, Tokyo 101-8437, Japan. kumagai@kyoritsu-wu.ac.jp.
⁶ Department of Chemistry and Life Science, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan. kumagai@brs.nihon-u.ac.jp.

Abstract

Phytate-removed and deamidated soybean β-conglycinin (PrDS) prepared by ion-exchange resins was supplemented to be 4% in the diet administered to ovariectomized rats to investigate its preventive effect on osteoporosis. The apparent calcium absorption rate decreased following ovariectomy and was not replenished by oral administration of phytate-removed soybean β-conglycinin (PrS) or casein. On the other hand, administration of PrDS restored the calcium absorption rate to the same level as the sham group. Markers of bone resorption, such as serum parathyroid hormone (PTH) and urinary deoxypyridinoline (DPD), increased, and the bone mineral density and breaking stress decreased following ovariectomy. However, PrDS supplementation suppressed the changes caused by the decrease in calcium absorption from the small intestine. Therefore, PrDS supplementation shows promise for the prevention of postmenopausal osteoporosis.

MeSH terms

Absorption, Physiological / drug effects
Administration, Oral
Amides / isolation & purification*
Amino Acids / urine
Animals
Antigens, Plant / administration & dosage*
Antigens, Plant / pharmacology
Antigens, Plant / therapeutic use*
Biomechanical Phenomena / drug effects
Bone Density / drug effects
Bone and Bones / drug effects
Bone and Bones / physiopathology
Female
Globulins / administration & dosage*
Globulins / pharmacology
Globulins / therapeutic use*
Minerals / metabolism
Osteoporosis / blood
Osteoporosis / drug therapy*
Osteoporosis / prevention & control*
Osteoporosis / urine
Ovariectomy
Parathyroid Hormone / blood
Phytic Acid / isolation & purification*
Rats, Wistar
Seed Storage Proteins / administration & dosage*
Seed Storage Proteins / pharmacology
Seed Storage Proteins / therapeutic use*
Soybean Proteins / administration & dosage*
Soybean Proteins / pharmacology
Soybean Proteins / therapeutic use*

Substances

Amides
Amino Acids
Antigens, Plant
Globulins
Minerals
Parathyroid Hormone
Seed Storage Proteins
Soybean Proteins
beta-conglycinin protein, Glycine max
Phytic Acid
deoxypyridinoline