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1988 3
1989 5
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31,697 results

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Page 1
Showing results for guide li
Search for Ruide Li instead (2 results)
Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.
Tsuchida CA, Zhang S, Doost MS, Zhao Y, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JG. Tsuchida CA, et al. Mol Cell. 2022 Mar 17;82(6):1199-1209.e6. doi: 10.1016/j.molcel.2022.02.002. Epub 2022 Feb 25. Mol Cell. 2022. PMID: 35219382 Free PMC article.
A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a. Although it can induce RNA-guided genome editing in mammalian cells, the activity of CasX is …
A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuc …
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.
East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA. East-Seletsky A, et al. Nature. 2016 Oct 13;538(7624):270-273. doi: 10.1038/nature19802. Epub 2016 Sep 26. Nature. 2016. PMID: 27669025 Free PMC article.
In type VI systems, the single-subunit C2c2 protein functions as an RNA-guided RNA endonuclease (RNase). How this enzyme acquires mature CRISPR RNAs (crRNAs) that are essential for immune surveillance and how it carries out crRNA-mediated RNA cleavage remain unclear. ...Th …
In type VI systems, the single-subunit C2c2 protein functions as an RNA-guided RNA endonuclease (RNase). How this enzyme acquires mat …
Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens.
Morgens DW, Wainberg M, Boyle EA, Ursu O, Araya CL, Tsui CK, Haney MS, Hess GT, Han K, Jeng EE, Li A, Snyder MP, Greenleaf WJ, Kundaje A, Bassik MC. Morgens DW, et al. Nat Commun. 2017 May 5;8:15178. doi: 10.1038/ncomms15178. Nat Commun. 2017. PMID: 28474669 Free PMC article.
Here, to test potential solutions to this issue, we design and analyse a CRISPR-Cas9 library with 10 variable-length guides per gene and thousands of negative controls targeting non-functional, non-genic regions (termed safe-targeting guides), in addition to non-tar …
Here, to test potential solutions to this issue, we design and analyse a CRISPR-Cas9 library with 10 variable-length guides per gene …
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a.
Liu L, Li X, Ma J, Li Z, You L, Wang J, Wang M, Zhang X, Wang Y. Liu L, et al. Cell. 2017 Aug 10;170(4):714-726.e10. doi: 10.1016/j.cell.2017.06.050. Epub 2017 Jul 27. Cell. 2017. PMID: 28757251 Free article.
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. ...The crRNA-target RNA duplex binds in a positively charged central channel of the nuclease (NUC) lobe, and …
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential a …
Guide RNA library-based CRISPR screens in plants: opportunities and challenges.
Pan C, Li G, Bandyopadhyay A, Qi Y. Pan C, et al. Curr Opin Biotechnol. 2023 Feb;79:102883. doi: 10.1016/j.copbio.2022.102883. Epub 2023 Jan 3. Curr Opin Biotechnol. 2023. PMID: 36603502 Review.
However, genetic screening at a large or genomic scale remains challenging in plants. Recently, the RNA-guided CRISPR-Cas nucleases have been optimized for high-throughput functional genomic screens combined with guide RNA (gRNA) libraries in plants. ...
However, genetic screening at a large or genomic scale remains challenging in plants. Recently, the RNA-guided CRISPR-Cas nucleases h …
Clinical applications and prospects of 3D printing guide templates in orthopaedics.
Meng M, Wang J, Sun T, Zhang W, Zhang J, Shu L, Li Z. Meng M, et al. J Orthop Translat. 2022 May 13;34:22-41. doi: 10.1016/j.jot.2022.03.001. eCollection 2022 May. J Orthop Translat. 2022. PMID: 35615638 Free PMC article. Review.
METHODS: In this paper, a systematic review of the orthopedic guide template is presented, where the history of 3D-printing-guided technology, the process of guides, and basic clinical applications of orthopedic guide templates are described. ...With t …
METHODS: In this paper, a systematic review of the orthopedic guide template is presented, where the history of 3D-printing-guided …
Elucidating the Role of Rational Separator Microstructures in Guiding Dendrite Growth and Reviving Dead Li.
Gao LT, Huang P, Guo ZS. Gao LT, et al. ACS Appl Mater Interfaces. 2022 Sep 21;14(37):41957-41968. doi: 10.1021/acsami.2c09551. Epub 2022 Sep 7. ACS Appl Mater Interfaces. 2022. PMID: 36069725
Herein, the effects of tuning the porous separator microstructure (SM) for guiding Li dendrite growth and reviving dead Li are revealed using a mechano-electrochemical phase-field model. A strategy of guiding, instead of suppression, was applied to avo …
Herein, the effects of tuning the porous separator microstructure (SM) for guiding Li dendrite growth and reviving dead Li …
Patient-Specific Implants in Orthognathic Surgery.
Li DTS, Leung YY. Li DTS, et al. Oral Maxillofac Surg Clin North Am. 2023 Feb;35(1):61-69. doi: 10.1016/j.coms.2022.06.004. Epub 2022 Nov 3. Oral Maxillofac Surg Clin North Am. 2023. PMID: 36336593 Review.
Virtual surgical planning and three-dimensional (3D) printing have broadened the horizons of oral and maxillofacial surgery, including orthognathic surgery. 3D-printed personalized surgical guide and patient-specific implant (PSI) not only serve to guide accurate os …
Virtual surgical planning and three-dimensional (3D) printing have broadened the horizons of oral and maxillofacial surgery, including ortho …
Patient-Derived Organoid Pharmacotyping Guides Precision Medicine for Pancreatic Cancer.
Zhang Y, Houchen CW, Li M. Zhang Y, et al. Clin Cancer Res. 2022 Aug 2;28(15):3176-3178. doi: 10.1158/1078-0432.CCR-22-1083. Clin Cancer Res. 2022. PMID: 35617521 Free PMC article.
High-throughput drug screening in terms of pharmacotyping using standard-of-care chemotherapy agents in the PDO platform has shown promising sensitivities to guide precision medicine for individual patients with pancreatic ductal adenocarcinoma (PDAC) within a clinically r …
High-throughput drug screening in terms of pharmacotyping using standard-of-care chemotherapy agents in the PDO platform has shown promising …
Renal Tumor Biopsy Technique.
Zhang L, Li XS, Zhou LQ. Zhang L, et al. Chin Med J (Engl). 2016 May 20;129(10):1236-40. doi: 10.4103/0366-6999.181957. Chin Med J (Engl). 2016. PMID: 27174334 Free PMC article. Review.
RESULTS: Computed tomography and ultrasound were usually used for guiding RTB with respective advantages. Core biopsy is more preferred over fine needle aspiration because of superior accuracy. A minimum of two good-quality cores for a single renal tumor is generally accep …
RESULTS: Computed tomography and ultrasound were usually used for guiding RTB with respective advantages. Core biopsy is more preferr …
31,697 results
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