Survey for Major Grapevine Viruses in Commercial Vineyards of Northwestern Argentina

Mónica Rivadeneira; Marta Zulema Galván; Marina Abán; Rosa Elena Semke; Josefina Rivadeneira; Melisa Lanza Volpe; Sebastián Gomez Talquenca

doi:10.3390/plants11131720

Survey for Major Grapevine Viruses in Commercial Vineyards of Northwestern Argentina

Plants (Basel). 2022 Jun 28;11(13):1720. doi: 10.3390/plants11131720.

Authors

Mónica Rivadeneira¹, Marta Zulema Galván^{1

2}, Marina Abán^{1

2}, Rosa Elena Semke³, Josefina Rivadeneira^{1

2}, Melisa Lanza Volpe⁴, Sebastián Gomez Talquenca⁴

Affiliations

¹ Instituto Nacional de Tecnología Agropecuaria (INTA) EEA Salta, Ruta Nacional 68 Km 172 (4403) Cerrillos, Salta 4403, Argentina.
² Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) CCT-Salta, J.M. Leguizamón 366, Salta 4400, Argentina.
³ Centro de Desarrollo Vitícola del Valle Calchaquí, Cafayate, Salta 4427, Argentina.
⁴ Instituto Nacional de Tecnología Agropecuaria (INTA) EEA, Mendoza 5602, Argentina.

Abstract

This study aimed to survey the occurrence of eight grapevine viruses in commercial vineyards located in the Calchaquíes Valleys in the northwest region of Argentina. A total of 103 samples of mature canes of vines showing either none or some viral-like symptoms were randomly collected. The samples were tested by RT-PCR/PCR-based assays for the screening of the following viruses: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated viruses (GLRaV-1, -2, -3, -4), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated viruses (GRSPaV), and Grapevine red blotch virus (GRBV). Sixty percent of the analyzed samples showed infection with some of the analyzed viruses, except GRBV. GLRaV-3 and GFLV were the most frequent viruses, present in 34% and 21% of the positive samples, respectively. This study represents the first survey report of the presence of grapevine viruses in the region of the Calchaquíes Valleys and contributes to the knowledge to maintain the sanitary status of commercial vineyards in Argentina.

Keywords: Calchaquíes Valleys; Vitis vinifera; grapevine viruses.

Grants and funding

This research was funded by Instituto Nacional de Tecnología Agropecuaria, grant numbers PDI090, PDI081 and PEI074. M.A. was supported by a doctoral scholarship from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.