Formation of Fibrils by the Periplasmic Molecular Chaperone HdeB from Escherichia coli

Int J Mol Sci. 2022 Oct 31;23(21):13243. doi: 10.3390/ijms232113243.

Abstract

The molecular chaperones HdeA and HdeB of the Escherichia coli (E. coli) periplasm protect client proteins from acid denaturation through a unique mechanism that utilizes their acid denatured states to bind clients. We previously demonstrated that the active, acid-denatured form of HdeA is also prone to forming inactive, amyloid fibril-like aggregates in a pH-dependent, reversible manner. In this study, we report that HdeB also displays a similar tendency to form fibrils at low pH. HdeB fibrils were observed at pH < 3 in the presence of NaCl. Similar to HdeA, HdeB fibrils could be resolubilized by a simple shift to neutral pH. In the case of HdeB, however, we found that after extended incubation at low pH, HdeB fibrils were converted into a form that could not resolubilize at pH 7. Fresh fibrils seeded from these “transformed” fibrils were also incapable of resolubilizing at pH 7, suggesting that the transition from reversible to irreversible fibrils involved a specific conformational change that was transmissible through fibril seeds. Analyses of fibril secondary structure indicated that HdeB fibrils retained significant alpha helical content regardless of the conditions under which fibrils were formed. Fibrils that were formed from HdeB that had been treated to remove its intrinsic disulfide bond also were incapable of resolubilizing at pH 7, suggesting that certain residual structures that are retained in acid-denatured HdeB are important for this protein to recover its soluble state from the fibril form.

Keywords: HdeB; amyloid fibril; denaturation; molecular chaperone; periplasm.

MeSH terms

  • Acids / metabolism
  • Amyloid / chemistry
  • Amyloid / metabolism
  • Escherichia coli Proteins* / metabolism
  • Escherichia coli* / metabolism
  • Humans
  • Hydrogen-Ion Concentration
  • Molecular Chaperones* / metabolism
  • Periplasm / metabolism
  • Protein Structure, Secondary

Substances

  • Acids
  • Amyloid
  • Escherichia coli Proteins
  • hdeA protein, E coli
  • Molecular Chaperones
  • hdeB protein, E coli