Novel mounting of biological tissue samples for 3D model reconstruction using tandem scanning electron microscopy and photogrammetry software

Biological 3D models have a multitude of applications in both research and academic settings, however the generation of such models at an ultrastructural scale has remained a daunting task. Here our group presents a method by which ultrastructural 3D models can be generated using tandem scanning electron microscopy (SEM) and photogrammetry. Our methods include a novel technique for mounting specimens for SEM which allowed our group to capture images from all angles around the specimen. Our results demonstrate that using our technique is adequate for the construction of an interactive, ultrastructural 3D model that can be viewed from all orientations. We ultimately see use for these models in educational settings and research when the 3D analysis of ultrastructural anatomy is necessary. HIGHLIGHTS: Scanning electron microscopy (SEM) was used in tandem with photogrammetry to generate an ultrastructural three-dimensional model of a whole zebrafish brain. A novel mounting technique for samples in SEM was used to view the sample from all angles. This technique can be applied to a wide range of samples for 3D model generation for use in several settings, including research and academia.