Effect of Reduction Methods on the Properties of Composite Films of Bacterial Cellulose-Silver Nanoparticles

Composite films of bacterial cellulose-silver nanoparticles (BC-Ag) were prepared by different methods of in situ reduction of silver ions, using sodium hydroxide, ascorbic acid, chitosan, and UV irradiation. The effects of the reduction methods on their properties were investigated. The chitosan-reduced composite exhibited dispersed silver nanoparticles (AgNPs) within the nanocellulose matrix with the smallest size, while the ascorbic-reduced composite displayed the largest size. The incorporation of AgNPs tended to reduce the crystallinity of the composites, except for the ascorbic-reduced composite, which exhibited an increase in crystallinity. Mechanical testing revealed that the ascorbic-reduced composite had the highest Young's modulus of 8960 MPa, whereas the UV-reduced composite had the highest tensile strength and elongation at break. Thermal analysis of BC-Ag composites indicated similar glass transition temperature and decomposition profiles to BC, with additional weight-loss steps at high temperatures. The sodium hydroxide-reduced composite demonstrated the highest electrical conductivity of 1.1 × 10^-7 S/cm. Water absorption capacity was reduced by the incorporation of AgNPs, except for the chitosan-reduced composite, which showed an enhanced water absorption capacity of 344%. All BC-Ag composites displayed very strong antibacterial activities against Staphylococcus aureus and Escherichia coli. These results also highlight the potential uses of BC-Ag composites for various applications.