Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

Olga A Krasheninina; Evgeny K Apartsin; Elena Fuentes; Aleksandra Szulc; Maksim Ionov; Alya G Venyaminova; Dzmitry Shcharbin; F Javier de la Mata; Maria Bryszewska; Rafael Gόmez

doi:10.3390/pharmaceutics11010025

Complexes of Pro-Apoptotic siRNAs and Carbosilane Dendrimers: Formation and Effect on Cancer Cells

Pharmaceutics. 2019 Jan 10;11(1):25. doi: 10.3390/pharmaceutics11010025.

Authors

Olga A Krasheninina¹, Evgeny K Apartsin², Elena Fuentes^{3

4}, Aleksandra Szulc⁵, Maksim Ionov⁶, Alya G Venyaminova⁷, Dzmitry Shcharbin⁸, F Javier de la Mata^{9

10

11}, Maria Bryszewska¹², Rafael Gόmez^{13

14

15}

Affiliations

¹ Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia. okrasheninina@gmail.com.
² Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia. eka@niboch.nsc.ru.
³ Departamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, Spain. elena.fuentes.paniagua@gmail.com.
⁴ Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain. elena.fuentes.paniagua@gmail.com.
⁵ Department of General Biophysics, University of Lodz, 90-236 Lodz, Poland. aleksandra_szulc@interia.pl.
⁶ Department of General Biophysics, University of Lodz, 90-236 Lodz, Poland. maksion@biol.uni.lodz.pl.
⁷ Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia. ven@niboch.nsc.ru.
⁸ Institute of Biophysics and Cell Engineering of NASB, 220072 Minsk, Belarus. d.shcharbin@gmail.com.
⁹ Departamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, Spain. javier.delamata@uah.es.
¹⁰ Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain. javier.delamata@uah.es.
¹¹ Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Madrid, Spain. javier.delamata@uah.es.
¹² Department of General Biophysics, University of Lodz, 90-236 Lodz, Poland. marbrys@biol.uni.lodz.pl.
¹³ Departamento de Química Orgánica y Química Inorgánica, UAH-IQAR, Universidad de Alcalá, 28805 Alcalá de Henares, Spain. rafael.gomez@uah.es.
¹⁴ Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain. rafael.gomez@uah.es.
¹⁵ Instituto Ramón y Cajal de Investigación Sanitaria, IRYCIS, Madrid, Spain. rafael.gomez@uah.es.

Abstract

This paper examines the complexation of anti-cancer small interfering RNAs (siRNAs) by cationic carbosilane dendrimers, and the interaction of the formed complexes with HeLa and HL-60 cancer cells. Stepwise formation of the complexes accompanied by the evolution of their properties has been observed through the increase of the charge ratio (dendrimer/siRNA). The complexes decrease the viability of both "easy-to-transfect" cells (HeLa) and "hard-to transfect" ones (HL-60), indicating a high potential of the cationic carbosilane dendrimers for siRNA delivery into tumor cells.

Keywords: anticancer siRNA; biophysical assays; carbosilane; complexation; cytotoxicity; dendrimers; dendriplexes; nucleic acids; transfection.

Abstract

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