Immobilizing Laccase on Modified Cellulose/CF Beads to Degrade Chlorinated Biphenyl in Wastewater

Na Li; Quiyang Xia; Yuan Li; Xiaobang Hou; Meihong Niu; Qingwei Ping; Huining Xiao

doi:10.3390/polym10070798

Immobilizing Laccase on Modified Cellulose/CF Beads to Degrade Chlorinated Biphenyl in Wastewater

Polymers (Basel). 2018 Jul 19;10(7):798. doi: 10.3390/polym10070798.

Authors

Na Li^{1

2

3}, Quiyang Xia⁴, Yuan Li⁵, Xiaobang Hou⁶, Meihong Niu⁷, Qingwei Ping⁸, Huining Xiao⁹

Affiliations

¹ Liaoning Province Key Laboratory of Plup and Papermaking Engineering, Dalian Polytechnic University, Dalian 116034, China. lina@dlpu.edu.cn.
² Key Laboratory of Pulp and Paper Science & Technology of Ministry of Education/Shandong Province, Qilu University of Technology, Jinan 250353, China. lina@dlpu.edu.cn.
³ Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B 5A3, Canada. lina@dlpu.edu.cn.
⁴ Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B 5A3, Canada. qxia2@unb.ca.
⁵ Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B 5A3, Canada. yli7@unb.ca.
⁶ Department of Environment Science & Engineering, North China Electric Power University, Baoding 071003, China. xhou1@unb.ca.
⁷ Liaoning Province Key Laboratory of Plup and Papermaking Engineering, Dalian Polytechnic University, Dalian 116034, China. niumh@dlpu.edu.cn.
⁸ Liaoning Province Key Laboratory of Plup and Papermaking Engineering, Dalian Polytechnic University, Dalian 116034, China. pingqw@dlpu.edu.cn.
⁹ Department of Chemical Engineering, University of New Brunswick, Fredericton, NB E3B 5A3, Canada. hxiao@unb.ca.

Abstract

Novel modified cellulose/cellulose fibril (CF) beads (MCCBs) loaded with laccase were prepared to degrade polychlorinated biphenyls (PCBs) in wastewater. The proper porous structure in MCCBs was achieved by introducing nano CaCO₃ (as a pore forming agent) in cellulose/CF (CCBs) beads during the preparation process. Cellulose/CF composite beads were modified by maleic anhydride to introduce carboxyl groups. Laccase was immobilized on the MCCBs through electrostatic adsorption and covalent bonding. The effects of pH, laccase concentration and contact time on immobilization yields and recovered activity were investigated. The best conditions were pH 4, concentration 16 g/L and contact time 3 h. The immobilized laccase under these conditions showed a good performance in thermal and operational stability. The laccase immobilized on MCCB beads can remove 85% of 20 mg/L 4-hydroxy-3,5-dichlorobiphenyl (HO-DiCB) in wastewater. The results demonstrated that MCCBs, as a new type of green-based support, are very promising in material immobilizing laccase. This technology may be of potential advantage for the removal of polychlorinated biphenyls in wastewater from an environmental point of view.

Keywords: adsorption; cellulose/CF beads; chlorinated biphenyl pollutants; laccase immobilization.

Abstract

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