Nanoemulsion containing Yellow Monascus pigment : Fabrication, characterization, storage stability, and lipase hydrolytic activity in vitro digestion

Kexin Li; Zhenlong Guo; Hui Li; Xueyong Ren; Changxia Sun; Quandong Feng; Shunli Kou; Qiang Li

doi:10.1016/j.colsurfb.2023.113199

Nanoemulsion containing Yellow Monascus pigment : Fabrication, characterization, storage stability, and lipase hydrolytic activity in vitro digestion

Colloids Surf B Biointerfaces. 2023 Apr:224:113199. doi: 10.1016/j.colsurfb.2023.113199. Epub 2023 Feb 12.

Authors

Kexin Li¹, Zhenlong Guo¹, Hui Li¹, Xueyong Ren², Changxia Sun³, Quandong Feng⁴, Shunli Kou⁵, Qiang Li⁶

Affiliations

¹ Department of Chemistry, College of Science, Beijing Forestry University, Beijing 100083, China; Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
² College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China. Electronic address: rxueyong@bjfu.edu.cn.
³ Department of Chemistry, College of Science, Beijing Forestry University, Beijing 100083, China; Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: susansun@bjfu.edu.cn.
⁴ Department of Chemistry, College of Science, Beijing Forestry University, Beijing 100083, China.
⁵ Zhejiang University of Science & Technology, Zhejiang 310023, China.
⁶ Department of Chemistry, College of Science, Beijing Forestry University, Beijing 100083, China; Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: liqiang@bjfu.edu.cn.

PMID: 36801744
DOI: 10.1016/j.colsurfb.2023.113199

Abstract

The natural pigment of monascus is favored by human for its special coloring and physiological activity, and its development and application have attracted much attention. In this study, a novel corn oil-based nanoemulsion encapsulated with Yellow Monascus Pigment crude extract (CO-YMPN) was successfully prepared via the phase inversion composition method. The fabrication and stable conditions of the CO-YMPN including Yellow Monascus pigment crude extract (YMPCE) concentration, emulsifier ratio, pH, temperature, ionic strength, monochromatic light and storage time were investigated systemically. The optimized fabrication conditions were the emulsifier ratio (5:3 ratio of Tween 60 to Tween 80) and the YMPCE concentration (20.00% wt%)). Additionally, the DPPH radical scavenging capability of the CO-YMPN (19.47 ± 0.52%) was more excellent than each YMPCE or corn oil. Moreover, the kinetic analysis results based on Michaelis-Menten equation and constant revealed that CO-YMPN could improve lipase hydrolysis capacity. Therefore, the CO-YMPN complex had excellent storage stability and water solubility in the final water system, and the YMPCE showed brilliant stability.

Keywords: Corn oil; DPPH; Michaelis-Menten equation; Nanoemulsion; Yellow Monascus Pigment.

MeSH terms

Corn Oil
Digestion
Emulsifying Agents
Humans
Hydrolysis
Kinetics
Lipase
Monascus* / chemistry
Pigments, Biological* / chemistry
Polysorbates
Water

Substances

Pigments, Biological
Corn Oil
Polysorbates
Emulsifying Agents
Water
Lipase