Objective: To investigate the clinical significance of cell blocks obtained by ultrasound-guided fine needle aspiration in diagnosing parotid gland masses.
Methods: Cell blocks were made in 285 parotid gland masses by ultrasound-guided fine needle aspiration. Diagnosis was conducted using the cell blocks. Non-tumor masses were subjected to conservative treatment, and cysts and tumors were treated with surgery. The cell block sections from masses with the diagnosis of adenoid cystic carcinoma (ACC) and pleomorphic adenoma (PA) were applied to the detection of immunocytochemical staining for the stem cell factor receptor CD117.
Results: The satisfaction rate of the specimen was 95.1% (271/285). The accuracy rate of the diagnosis was 94.5% (256/271), the sensitivity was 87.0% (67/77), and the specificity was 98.1% (157/160). The positive rate of CD117 in ACC was 95.2% (20/21), whereas that in PA was 20.3% (25/123). The positive rate of CD117 in ACC was higher than that in PA (P<0.01).
Conclusions: The use of cell blocks obtained from ultrasound-guided fine needle aspiration, together with molecular marker detection, has great significance in diagnosing parotid gland masses. .
目的 探讨超声引导下细针穿刺制作细胞块在诊断腮腺区肿块方面的应用价值。方法 在彩色超声仪引导下，对285个腮腺区肿块进行细针穿刺，将穿刺标本制成细胞块，以进行病理学诊断。对非肿瘤性肿块采取保守治疗，囊肿与肿瘤性肿块采取手术治疗。对术后病理确诊为腺样囊性癌（ACC）与多形性腺瘤（PA）对应的细胞块行干细胞因子受体CD117免疫组织化学检测。结果 标本制作满意率为 95.1%（271/285），诊断准确率为 94.5%（256/271）；诊断敏感度为87.0%（67/77），特异度为98.1%（157/160）。CD117在ACC中的阳性表达率为95.2%（20/21），在PA中为20.3%（25/123），ACC中阳性表达率明显高于PA，二者差异有统计学意义（P<0.01）。结论 超声引导下腮腺区肿块细针穿刺制作细胞块结合分子标志物检测，对腮腺区肿块的诊断具有重要意义。.
Keywords: cell block; fine needle aspiration; mass; molecular marker; parotid gland.