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Showing results for philipp reporter
Search for Philipp Rapolter instead (1 results)
The Wales Endoscope: The First American Cystoscope.
Patel SR, Moran ME, Rugendorff EW, Rabinowitz R. Patel SR, et al. Urology. 2022 Dec;170:1-4. doi: 10.1016/j.urology.2022.07.041. Epub 2022 Aug 11. Urology. 2022. PMID: 35964785
INTRODUCTION: Philipp Bozzini, a German army surgeon, in 1807 invented the Lichtleiter, the predecessor of the modern cystoscope. ...In 1868 he published a series of papers in the Philadelphia Medical and Surgical Reporter on "Instrumental Diagnosis," with a paper e …
INTRODUCTION: Philipp Bozzini, a German army surgeon, in 1807 invented the Lichtleiter, the predecessor of the modern cystoscope. ... …
High-throughput functional analysis of regulatory variants using a massively parallel reporter assay.
Delfosse K, Gerhardinger C, Rinn JL, Maass PG. Delfosse K, et al. STAR Protoc. 2023 Dec 15;4(4):102731. doi: 10.1016/j.xpro.2023.102731. Epub 2023 Nov 18. STAR Protoc. 2023. PMID: 37980569 Free PMC article.
We present a protocol to assay the regulatory potential of thousands of noncoding variants using massively parallel reporter assays. We describe steps for oligo design, generating a plasmid pool, and extracting tag-seq libraries from cells to quantify the tested sequences. …
We present a protocol to assay the regulatory potential of thousands of noncoding variants using massively parallel reporter assays. …
Isoform-specific translational control is evolutionarily conserved in primates.
Draper JM, Philipp J, Neeb ZT, Thomas R, Katzman S, Salama S, Haussler D, Sanford J. Draper JM, et al. bioRxiv [Preprint]. 2023 May 8:2023.04.21.537863. doi: 10.1101/2023.04.21.537863. bioRxiv. 2023. PMID: 37131629 Free PMC article. Preprint.
Accordingly, single nucleotide substitutions in luciferase reporters designed to model exons with divergent polyribosome profiles are sufficient to regulate translational efficiency. ...
Accordingly, single nucleotide substitutions in luciferase reporters designed to model exons with divergent polyribosome profiles are …
A Novel Potent Carrier for Unconventional Protein Export in Ustilago maydis.
Philipp M, Hussnaetter KP, Reindl M, Müntjes K, Feldbrügge M, Schipper K. Philipp M, et al. Front Cell Dev Biol. 2022 Jan 10;9:816335. doi: 10.3389/fcell.2021.816335. eCollection 2021. Front Cell Dev Biol. 2022. PMID: 35083222 Free PMC article.
Here, we show that Jps1 is unconventionally secreted using a fusion to bacterial beta-glucuronidase as an established reporter. Interestingly, the experiment also demonstrates that the protein functions as an alternative carrier for heterologous proteins, showing about 2-f …
Here, we show that Jps1 is unconventionally secreted using a fusion to bacterial beta-glucuronidase as an established reporter. Inter …
Intronic tRNAs of mitochondrial origin regulate constitutive and alternative splicing.
Hoser SM, Hoffmann A, Meindl A, Gamper M, Fallmann J, Bernhart SH, Müller L, Ploner M, Misslinger M, Kremser L, Lindner H, Geley S, Schaal H, Stadler PF, Huettenhofer A. Hoser SM, et al. Genome Biol. 2020 Dec 8;21(1):299. doi: 10.1186/s13059-020-02199-6. Genome Biol. 2020. PMID: 33292386 Free PMC article.
To address potential functions of intronic nimtRNAs, we insert them into introns of constitutive and alternative splicing reporters and demonstrate that nimtRNAs promote pre-mRNA splicing, dependent on the number and positioning of nimtRNA genes and splice site recognition …
To address potential functions of intronic nimtRNAs, we insert them into introns of constitutive and alternative splicing reporters a …
Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis.
Müntjes K, Philipp M, Hüsemann L, Heucken N, Weidtkamp-Peters S, Schipper K, Zurbriggen MD, Feldbrügge M. Müntjes K, et al. Front Microbiol. 2020 Jun 24;11:1384. doi: 10.3389/fmicb.2020.01384. eCollection 2020. Front Microbiol. 2020. PMID: 32670239 Free PMC article.
Here, we establish such a system in the well-studied model microorganism Ustilago maydis. Using two fluorescence reporter proteins, we compared the activity of five viral 2A peptides. ...
Here, we establish such a system in the well-studied model microorganism Ustilago maydis. Using two fluorescence reporter proteins, w …
Fluorescent tools for the standardized work in Gram-negative bacteria.
Delgadillo-Guevara M, Halte M, Erhardt M, Popp PF. Delgadillo-Guevara M, et al. J Biol Eng. 2024 Apr 8;18(1):25. doi: 10.1186/s13036-024-00420-9. J Biol Eng. 2024. PMID: 38589953 Free PMC article.
The discovery of fluorescent proteins (FPs) represents a milestone in the development of genetic reporters for monitoring transcription or protein localization in vivo. ...In summary, our study underscores the importance of careful FP selection and paves the way for the de …
The discovery of fluorescent proteins (FPs) represents a milestone in the development of genetic reporters for monitoring transcripti …
Volumetric HiLo microscopy employing an electrically tunable lens.
Philipp K, Smolarski A, Koukourakis N, Fischer A, Stürmer M, Wallrabe U, Czarske JW. Philipp K, et al. Opt Express. 2016 Jun 27;24(13):15029-41. doi: 10.1364/OE.24.015029. Opt Express. 2016. PMID: 27410654 Free article.
Proof of concept measurements at home-built specimens as well as zebrafish embryos with reporter gene-driven fluorescence in the thyroid gland are shown....
Proof of concept measurements at home-built specimens as well as zebrafish embryos with reporter gene-driven fluorescence in the thyr …
26 results