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Diversity Matters: Optimal Collision Energies for Tandem Mass Spectrometric Analysis of a Large Set of N-Glycopeptides.
Hevér H, Nagy K, Xue A, Sugár S, Komka K, Vékey K, Drahos L, Révész Á. Hevér H, et al. J Proteome Res. 2022 Nov 4;21(11):2743-2753. doi: 10.1021/acs.jproteome.2c00519. Epub 2022 Oct 6. J Proteome Res. 2022. PMID: 36201757 Free PMC article.
The resulting unprecedented amount of peptide-level information on such a large and diverse set of N-glycopeptides revealed that the peptide sequence heavily influences the energy for the highest score on top of an expected general linear trend with m/z. Search engine depe …
The resulting unprecedented amount of peptide-level information on such a large and diverse set of N-glycopeptides revealed that the peptide …
Tailoring to Search Engines: Bottom-Up Proteomics with Collision Energies Optimized for Identification Confidence.
Révész Á, Milley MG, Nagy K, Szabó D, Kalló G, Csősz É, Vékey K, Drahos L. Révész Á, et al. J Proteome Res. 2021 Jan 1;20(1):474-484. doi: 10.1021/acs.jproteome.0c00518. Epub 2020 Dec 7. J Proteome Res. 2021. PMID: 33284634 Free PMC article.
We thereby extended our earlier study on Mascot scores and corroborated its findings on the potential bimodal nature of this energy dependence. Optimal energies as a function of m/z show comparable linear trends for the three engines. On the basis of peptide-level results, …
We thereby extended our earlier study on Mascot scores and corroborated its findings on the potential bimodal nature of this energy dependen …
Collision energies on QTof and Orbitrap instruments: How to make proteomics measurements comparable?
Szabó D, Schlosser G, Vékey K, Drahos L, Révész Á. Szabó D, et al. J Mass Spectrom. 2021 Jan;56(1):e4693. doi: 10.1002/jms.4693. Epub 2020 Dec 5. J Mass Spectrom. 2021. PMID: 33277714
The fraction of bimodal peptides is lower on Orbitrap. Optimal energies as a function of m/z show a similar linear trend on both instruments, which suggests that with appropriate collision energy adjustment, matching conditions for proteomics can be achieved. ...
The fraction of bimodal peptides is lower on Orbitrap. Optimal energies as a function of m/z show a similar linear trend on both inst …
Can We Boost N-Glycopeptide Identification Confidence? Smart Collision Energy Choice Taking into Account Structure and Search Engine.
Hevér H, Xue A, Nagy K, Komka K, Vékey K, Drahos L, Révész Á. Hevér H, et al. J Am Soc Mass Spectrom. 2024 Feb 7;35(2):333-343. doi: 10.1021/jasms.3c00375. Epub 2024 Jan 29. J Am Soc Mass Spectrom. 2024. PMID: 38286027 Free PMC article.
We found that search engines show a range of behavior between peptide-centric and glycan-centric, which manifests itself already in the dependence of optimal collision energy on m/z. Using classical statistical and machine learning methods, we revealed that peptide hydroph …
We found that search engines show a range of behavior between peptide-centric and glycan-centric, which manifests itself already in the depe …