Recording fluorescence using flash photography, may help reduce time of capture and apply effectively in clinical practice. To test methods for visualizing composite resins fluorescence by direct digital photography. Sixty-four specimen discs (1.5 × 10 mm) were prepared from 8 different composite resin brands. Their CIELAB color coordinates (L*, a*, b*) and fluorescence were measured using a portable colorimeter and a fluorescence spectrophotometer. The mean of three measurements was recorded and then specimens were photographed by a DSLR camera with two different filters (365 nm and 405 nm) mounted on a commercial macro flash. RGB values of all specimens on the photographs were measured by using Photoshop software and converted to CIELab. Data were then analyzed using Kruskal-Wallis and Dunn's multiple comparisons tests. Correlation and regression analyses were also used to relate fluorescence and color parameters on the photographs at α = 0.05. Fluorescence and color data indicated significant differences among the materials (p < 0.05). L* a* and b* color coordinates from both photographs were highly correlated to fluorescence intensities found by the reference method (r365 - 0.95, r405 - 0.94), while regression analysis indicated a strong linear relationship (R2365 - 0.88, R2405 - 0.89). The study showed that filtered flash photography either by the use of a 365 nm or a 405 nm band pass filter can directly visualize fluorescence of composite resin materials and differences in fluorescence between them.
Keywords: Blue light fluorescence; Composite resin fluorescence; Direct fluorescence visualization; Photographic images; UVA light fluorescence.