Efficacy of Potentially Probiotic Fruit-Derived Lactobacillus fermentum, L. paracasei and L. plantarum to Remove Aflatoxin M₁ In Vitro

This study evaluated the efficacy of potentially probiotic fruit-derived Lactobacillus isolates, namely, L. paracasei 108, L. plantarum 49, and L. fermentum 111, to remove aflatoxin M₁ (AFM₁) from a phosphate buffer solution (PBS; spiked with 0.15 µg/mL AFM₁). The efficacy of examined isolates (approximately 10⁹ cfu/mL) as viable and non-viable cells (heat-killed; 100 °C, 1 h) to remove AFM₁ was measured after 1 and 24 h at 37 °C. The recovery of AFM₁ bound to bacterial cells after washing with PBS was also evaluated. Levels of AFM₁ in PBS were measured with high-performance liquid chromatography. Viable and non-viable cells of all examined isolates were capable of removing AFM₁ in PBS with removal percentage values in the range of 73.9-80.0% and 72.9-78.7%, respectively. Viable and non-viable cells of all examined Lactobacillus isolates had similar abilities to remove AFM₁. Only L. paracasei 108 showed higher values of AFM₁ removal after 24 h for both viable and non-viable cells. Percentage values of recovered AFM₁ from viable and non-viable cells after washing were in the range of 13.4-60.6% and 10.9-47.9%, respectively. L. plantarum 49 showed the highest AFM₁ retention capacity after washing. L. paracasei 108, L. plantarum 49, and L. fermentum 111 could have potential application to reduce AFM₁ to safe levels in foods and feeds. The cell viability of examined isolates was not a pre-requisite for their capacity to remove and retain AFM₁.