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Page 1
Showing results for P. titrant
Search for P. Titran instead (1 results)
Benzyl p-guanidinothiobenzoate hydrochloride, a new active-site titrant for trypsin and trypsin-like enzymes.
Cook RR, Powers JC. Cook RR, et al. Biochem J. 1983 Nov 1;215(2):287-94. doi: 10.1042/bj2150287. Biochem J. 1983. PMID: 6360155 Free PMC article.
Ellman's reagent has also been successfully employed, allowing measurement at 410 nm. Unlike p-nitrophenyl p'-guanidinobenzoate, the thioester titrant reacts slowly with chymotrypsin A alpha thus eliminating interference by this enzyme in most titrations. ... …
Ellman's reagent has also been successfully employed, allowing measurement at 410 nm. Unlike p-nitrophenyl p'-guanidinobenzoat …
Automated assay for alpha 1-antitrypsin with N-alpha-benzoyl-DL-arginine-p-nitroanilide as trypsin substrate and standardized with p-nitrophenyl-p'-guanidinobenzoate as titrant for trypsin active sites.
Eckfeldt JH, Light RT, Leiendecker-Foster C. Eckfeldt JH, et al. Clin Chem. 1982 May;28(5):1108-12. Clin Chem. 1982. PMID: 6280895
We describe an automated functional assay for the Instrumentation Laboratory's Multistat III Microcentrifugal Analyzer with N-alpha-benzoyl-DL-arginine-p-nitroanilide as trypsin substrate. The assay is standardized in terms of moles of trypsin active sites inhibited per li …
We describe an automated functional assay for the Instrumentation Laboratory's Multistat III Microcentrifugal Analyzer with N-alpha-benzoyl- …
Diethyl-p-nitrophenyl phosphate: an active site titrant for lipoprotein lipase.
Quinn DM. Quinn DM. Biochim Biophys Acta. 1985 Apr 25;834(2):267-71. doi: 10.1016/0005-2760(85)90165-1. Biochim Biophys Acta. 1985. PMID: 3995064
Diethyl-p-nitrophenyl phosphate is an active site-directed irreversible inhibitor of bovine milk lipoprotein lipase catalyzed hydrolysis of the water-soluble substrate, p-nitrophenyl butyrate. ...
Diethyl-p-nitrophenyl phosphate is an active site-directed irreversible inhibitor of bovine milk lipoprotein lipase catalyzed hydroly …
Stalagmometric titrations.
Kambara T, Kiba T. Kambara T, et al. Talanta. 1972 Apr;19(4):399-406. doi: 10.1016/0039-9140(72)80101-2. Talanta. 1972. PMID: 18961068
The drop-time of a polarographic dropping mercury electrode in open circuit is plotted against volume of surface-active titrant added, and shows a break or a peak at the end-point. The stalagmometric titration of sodium tetraphenylborate with Zephiramine (tetradecyldimethy …
The drop-time of a polarographic dropping mercury electrode in open circuit is plotted against volume of surface-active titrant added …
Human alpha-thrombin inhibition by the active site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azalysine p-nitrophenyl ester: a comparative kinetic and X-ray crystallographic study.
Nardini M, Pesce A, Rizzi M, Casale E, Ferraccioli R, Balliano G, Milla P, Ascenzi P, Bolognesi M. Nardini M, et al. J Mol Biol. 1996 May 24;258(5):851-9. doi: 10.1006/jmbi.1996.0292. J Mol Biol. 1996. PMID: 8637015
Kinetics for the hydrolysis of the chromogenic active site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azalysine p-nitrophenyl ester (Dmc-azaLys-ONp) catalyzed by bovine beta-trypsin, bovine alpha-thrombin, human alpha-thrombin, human Lys77-plasmin, human urinary …
Kinetics for the hydrolysis of the chromogenic active site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azalysine p-nitrophen …
N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p-nitrophenyl ester: a novel, high selective and optimal chromogenic active site titrant for human and bovine alpha-, beta- and gamma-thrombin.
Balliano G, Milla P, Giordano C, Gallina C, Coletta M, Menegatti E, Rizzi M, Bolognesi M, Ascenzi P. Balliano G, et al. Biochem Biophys Res Commun. 1996 Aug 14;225(2):557-61. doi: 10.1006/bbrc.1996.1211. Biochem Biophys Res Commun. 1996. PMID: 8753800
The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Phe-Pro-azaLys-ONp) was investigated at pH 6.2 and 21.0 degrees C. ...Eoc-D-Phe- Pro-azaLys-ONp shows the characteristics of a novel, high sel …
The serine proteinase catalyzed hydrolysis of N-ethoxycarbonyl-D-phenylalanyl-L-prolyl-alpha-azalysine p- nitrophenyl ester (Eoc-D-Ph …
Serine proteinase inhibition by the active site titrant N alpha-(N, N-dimethylcarbamoyl)-alpha-azaornithine p-nitrophenyl ester. A comparative study.
Ascenzi P, Balliano G, Gallina C, Polticelli F, Bolognesi M. Ascenzi P, et al. Eur J Biochem. 2000 Feb;267(4):1239-46. doi: 10.1046/j.1432-1327.2000.01120.x. Eur J Biochem. 2000. PMID: 10672036 Free article.
Kinetics for the hydrolysis of the chromogenic active-site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azaornithine p-nitrophenyl ester (Dmc-azaOrn-ONp) catalysed by bovine beta-trypsin, bovine alpha-thrombin, bovine Factor Xa, human alpha-thrombin, human Factor X …
Kinetics for the hydrolysis of the chromogenic active-site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azaornithine p-nitrop …
(p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases.
Laura R, Robison DJ, Bing DH. Laura R, et al. Biochemistry. 1980 Oct 14;19(21):4859-64. doi: 10.1021/bi00562a024. Biochemistry. 1980. PMID: 6775696
In contrast, p-APMSF in large molar excess does not inactivate chymotrypsin or acetylcholinesterase. The unique reactivity of p-APMSF has been further shown in comparison with the related compound p-nitrophenyl (p-amidinophenyl)methanesulfonate which i …
In contrast, p-APMSF in large molar excess does not inactivate chymotrypsin or acetylcholinesterase. The unique reactivity of p
161 results