Synthetic biology based construction of biological activity-related library of fungal decalin-containing diterpenoid pyrones.
Tsukada K, Shinki S, Kaneko A, Murakami K, Irie K, Murai M, Miyoshi H, Dan S, Kawaji K, Hayashi H, Kodama EN, Hori A, Salim E, Kuraishi T, Hirata N, Kanda Y, Asai T.
Tsukada K, et al.
Nat Commun. 2020 Apr 14;11(1):1830. doi: 10.1038/s41467-020-15664-4.
Nat Commun. 2020.
PMID: 32286350
Free PMC article.
In total, ten DDP pathways, including five native pathways, four extended pathways and one shunt pathway, are heterologously reconstituted in a genetically tractable heterologous host, Aspergillus oryzae, resulting in the production of 22 DDPs, including 15 new analogues. …
In total, ten DDP pathways, including five native pathways, four extended pathways and one shunt pathway, are heterologously reconstituted i …