Supramolecular structure of banana peel pectin and its transformations during extraction by acidic methods

In this study, the approaches to describe the mesh structure in the homogalacturonate domains of pectin and the effect of the native structure violations on the stabilization effectiveness of the oil-in-water emulsion were demonstrated. Pectin with a native structure was isolated from banana peel by enzymolysis of insoluble dietary fibres. This pectin was compared with pectins, which were isolated using hydrochloric and citric acids. The properties of pectins were analyzed taking into account the ratio of galacturonate units in nonsubstituted, methoxylated and calcium-pectate forms. The content of calcium-pectate units determines the density of inter-molecular crosslinking formation. The simulation results reflect the structure of rigid "egg-box" crosslinking blocks and flexible segments formed in native pectin mainly by methoxylated links. Hydrochloric acid extraction is accompanied by the destruction of the crosslinking blocks and depolymerization of pectin. Citric acid partially demineralizes the crosslinking blocks contributing to the release of macromolecular chains that do not have calcium-pectate units. The granulometric data indicates that the individual macromolecules take the thermodynamically stable form of a statistical tangle. Such conformation is an ideal basis for the formation of "host-guest" microcontainers having a hydrophilic shell and a hydrophobic core with an oil-soluble functional substance.