Abstract
The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg²⁺ as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide.
Keywords:
Algoa bay; Bacillus sp. Gilbert; bioflocculant; marine; polysaccharide.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Ammonium Chloride / chemistry
-
Ammonium Chloride / metabolism
-
Bacillus / isolation & purification
-
Bacillus / metabolism*
-
Bacillus / physiology
-
Bacteria / isolation & purification
-
Bacteria / metabolism
-
Bacterial Proteins / chemistry
-
Bacterial Proteins / metabolism*
-
Base Sequence
-
Bays
-
Carbon
-
Culture Media
-
Flocculation
-
Geologic Sediments / microbiology
-
Hydrogen-Ion Concentration
-
Nitrogen
-
Polymers / chemistry
-
Polymers / metabolism*
-
Polysaccharides / analysis
-
Polysaccharides / chemistry*
-
RNA, Ribosomal, 16S / analysis
-
RNA, Ribosomal, 16S / genetics
-
South Africa
-
Sucrose / chemistry
-
Sucrose / metabolism
-
Time Factors
Substances
-
Bacterial Proteins
-
Culture Media
-
Polymers
-
Polysaccharides
-
RNA, Ribosomal, 16S
-
Ammonium Chloride
-
Sucrose
-
Carbon
-
Nitrogen