Development of a PrGO-Modified Electrode for Uric Acid Determination in the Presence of Ascorbic Acid by an Electrochemical Technique

Nurulkhalilah Tukimin; Jaafar Abdullah; Yusran Sulaiman

doi:10.3390/s17071539

Development of a PrGO-Modified Electrode for Uric Acid Determination in the Presence of Ascorbic Acid by an Electrochemical Technique

Sensors (Basel). 2017 Jul 1;17(7):1539. doi: 10.3390/s17071539.

Authors

Nurulkhalilah Tukimin¹, Jaafar Abdullah^{2

3}, Yusran Sulaiman^{4

5}

Affiliations

¹ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia. nurulkhalilah.tukimin@gmail.com.
² Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia. jafar@upm.edu.my.
³ Institute of Advanced Technology (ITMA), Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia. jafar@upm.edu.my.
⁴ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia. yusran@upm.edu.my.
⁵ Institute of Advanced Technology (ITMA), Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia. yusran@upm.edu.my.

Abstract

An attractive electrochemical sensor of poly(3,4-ethylenedioxythiophene)/reduced graphene oxide electrode (PrGO) was developed for an electrochemical technique for uric acid (UA) detection in the presence of ascorbic acid (AA). PrGO composite film showed an improved electrocatalytic activity towards UA oxidation in pH 6.0 (0.1 M PBS). The PrGO composite exhibited a high current signal and low charge transfer resistance (R_ct) compared to a reduced graphene oxide (rGO) electrode or a bare glassy carbon electrode (GCE). The limit of detection and sensitivity of PrGO for the detection of UA are 0.19 μM (S/N = 3) and 0.01 μA/μM, respectively, in the range of 1-300 μM of UA.

Keywords: analytical sensor; differential pulse voltammetry; electrochemistry; poly(3,4-ethylenedioxythiophene); reduced graphene oxide; uric acid.