Biocompatibility and biological activity of C70 fullerene adduct with L-threonine (C70(C4H9NO3)2)

Vladimir V Sharoyko; Evgenii B Serebriakov; Sergei V Ageev; Andrey V Petrov; Anatolii A Meshcheriakov; Nikolay A Charykov; Igor V Murin; Dmitrii N Maistrenko; Oleg E Molchanov; Konstantin N Semenov

doi:10.1016/j.bbrc.2022.10.054

Biocompatibility and biological activity of C₇₀ fullerene adduct with L-threonine (C₇₀(C₄H₉NO₃)₂)

Biochem Biophys Res Commun. 2022 Dec 25;636(Pt 1):50-56. doi: 10.1016/j.bbrc.2022.10.054. Epub 2022 Oct 19.

Affiliations

¹ Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo Str., Saint Petersburg, 197022, Russia; Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Pr., Saint Petersburg, 198504, Russia; A. M. Granov Russian Research Centre for Radiology and Surgical Technologies, 70 Leningradskaia Str., Saint Petersburg, 197758, Russia. Electronic address: sharoyko@gmail.com.
² Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Pr., Saint Petersburg, 198504, Russia.
³ Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo Str., Saint Petersburg, 197022, Russia; Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Pr., Saint Petersburg, 198504, Russia.
⁴ Saint Petersburg State Technological Institute (Technical University), 26 Moskovskii Pr., Saint Petersburg, 190013, Russia.
⁵ A. M. Granov Russian Research Centre for Radiology and Surgical Technologies, 70 Leningradskaia Str., Saint Petersburg, 197758, Russia.
⁶ Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo Str., Saint Petersburg, 197022, Russia; Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii Pr., Saint Petersburg, 198504, Russia; A. M. Granov Russian Research Centre for Radiology and Surgical Technologies, 70 Leningradskaia Str., Saint Petersburg, 197758, Russia. Electronic address: knsemenov@gmail.com.

PMID: 36332482
DOI: 10.1016/j.bbrc.2022.10.054

Abstract

The aim of this work is to synthesise and study the biocompatibility and biological activity of the C₇₀ fullerene adduct with l-threonine (C₇₀-Thr). The obtained adduct was identified using a complex of physicochemical methods, namely, ¹³C NMR spectroscopy, IR spectroscopy, thermogravimetric analysis, electron spectroscopy, elemental analysis, and high-performance liquid chromatography. The study of biocompatibility and biological activity of the C₇₀-Thr adduct included the study of haemocompatibility (haemolysis, platelet aggregation, plasma coagulation haemostasis, binding to human serum albumin, esterase activity), antiradical activity, cytotoxicity, cell proliferation, and interaction with DNA (determination of the DNA binding constant and genotoxicity).

Keywords: Biocompatibility; Biological activity; DPPH; Fullerene; HSA; l-threonine.

MeSH terms

Chromatography, High Pressure Liquid
Fullerenes* / pharmacology
Humans
Magnetic Resonance Spectroscopy
Threonine

Substances

Fullerenes
Threonine