Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage

Mikhail S Bozhokin; Svetlana A Bozhkova; Aleksandr A Rubel; Julia V Sopova; Yulia A Nashchekina; Natalya B Bildyug; Mikhail G Khotin

doi:10.3390/mps4040077

Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage

Methods Protoc. 2021 Oct 27;4(4):77. doi: 10.3390/mps4040077.

Authors

Mikhail S Bozhokin^{1

2}, Svetlana A Bozhkova¹, Aleksandr A Rubel^{3

4

5}, Julia V Sopova^{2

3

5

6

7}, Yulia A Nashchekina², Natalya B Bildyug², Mikhail G Khotin²

Affiliations

¹ Vreden National Medical Research Center of Traumatology and Orthopedics, Academica Baykova Str., 8, 195427 St. Petersburg, Russia.
² Institute of Cytology, Russian Academy of Sciences, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia.
³ Laboratory of Amyloid Biology, St. Petersburg State University, Universitetskaja Emb., 7/9, 199034 St. Petersburg, Russia.
⁴ Department of Genetics and Biotechnology, St. Petersburg State University, Universitetskaja Emb., 7/9, 199034 St. Petersburg, Russia.
⁵ Research Center for Genetics and Life Sciences, Sirius University of Science and Technology, Olympiiskii ave., 1, 354340 Sochi, Russia.
⁶ Center of Transgenesis and Genome Editing, St. Petersburg State University, Universitetskaja Emb., 7/9, 199034 St. Petersburg, Russia.
⁷ Vavilov Institute of General Genetics, St. Petersburg Branch, Universitetskaja Emb., 7/9, 199034 St. Petersburg, Russia.

Abstract

Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.

Keywords: SEM; cellular engineered constructs; histology; hyaline cartilage; tissue engineering.

Abstract

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