In vivo tracking of plasmid DNA/mRNA: A novel labelling precursor for 89Zr positron emission tomography

Naohiro Aoyama; Sadaaki Kimura; Toshiyuki Matsui; Takashi Nozawa

doi:10.1016/j.bmcl.2023.129332

In vivo tracking of plasmid DNA/mRNA: A novel labelling precursor for ⁸⁹Zr positron emission tomography

Bioorg Med Chem Lett. 2023 Jun 15:90:129332. doi: 10.1016/j.bmcl.2023.129332. Epub 2023 May 15.

Authors

Naohiro Aoyama¹, Sadaaki Kimura², Toshiyuki Matsui², Takashi Nozawa²

Affiliations

¹ Astellas Pharma Inc., 21, Miyukigaoka, Tsukuba-shi, Ibaraki 305-8585, Japan. Electronic address: naohiro.aoyama@astellas.com.
² Astellas Pharma Inc., 21, Miyukigaoka, Tsukuba-shi, Ibaraki 305-8585, Japan.

PMID: 37196869
DOI: 10.1016/j.bmcl.2023.129332

Abstract

Herein, we developed a novel labelling precursor Fe-DFO-5 for plasmid DNA (pDNA) utilizing ⁸⁹Zr as a radioisotope for PET imaging. ⁸⁹Zr-labelled pDNA showed comparable gene expression to non-labelled pDNA. The biodistribution of ⁸⁹Zr-labelled pDNA after local or systemic administration in mice was evaluated. Furthermore, this labelling method was also applied to mRNA.

Keywords: (89)Zr; Biodistribution; Bioluminescence; Gene expression; Gene therapy; In vivo imaging; PET; Plasmid; Positron emission tomography; mRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line, Tumor
DNA
Mice
Plasmids / genetics
Positron-Emission Tomography* / methods
Tissue Distribution
Zirconium*

Substances

Zirconium
DNA