Tracking control of specific variables is key to achieve a proper fermentation. This paper analyzes a fed-batch bioethanol production process. For this system, a controller design based on linear algebra is proposed. Moreover, to achieve a reliable control, on-line monitoring of certain variables is needed. In this sense, for unmeasurable variables, state estimators based on Gaussian processes are designed. Cell, ethanol and glycerol concentrations are predicted with only substrates measurement. Simulation results when the controller and estimators are coupled, are shown. Furthermore, the algorithms were tested with parametric uncertainties and disturbances in the control action, and are compared, in all cases, with neural networks estimators (previous work). Bayesian estimators show a performance improvement, which is reflected in a decrease of the total error. Proposed techniques give reliable monitoring and control tools, with a low computational and economic cost, and less mathematical complexity than neural network estimators.
Keywords: Fed-batch bioprocess; Gaussian process; Non-linear and multivariable system; On-line monitoring; Profiles tracking control; State estimation.