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Molecular characterization of a new N-acetylneuraminate synthase (NeuB1) from Idiomarina loihiensis.
García García MI, Lau K, von Itzstein M, García Carmona F, Sánchez Ferrer Á. García García MI, et al. Glycobiology. 2015 Jan;25(1):115-23. doi: 10.1093/glycob/cwu096. Epub 2014 Sep 11. Glycobiology. 2015. PMID: 25214154
N-Acetylneuraminate lyase synthase (NeuB; E.C. 2.5.1.56) is a key enzyme in pathogenic microorganisms for producing N-acetylneuraminic acid through the irreversible condensation of N-acetylmannosamine (ManNAc) and phosphoenolpyruvate (PEP). ...The obta …
N-Acetylneuraminate lyase synthase (NeuB; E.C. 2.5.1.56) is a key enzyme in pathogenic microorganisms for producing N-a …
Structural and mechanistic analysis of sialic acid synthase NeuB from Neisseria meningitidis in complex with Mn2+, phosphoenolpyruvate, and N-acetylmannosaminitol.
Gunawan J, Simard D, Gilbert M, Lovering AL, Wakarchuk WW, Tanner ME, Strynadka NC. Gunawan J, et al. J Biol Chem. 2005 Feb 4;280(5):3555-63. doi: 10.1074/jbc.M411942200. Epub 2004 Oct 29. J Biol Chem. 2005. PMID: 15516336 Free article.
In Neisseria meningitidis and related bacterial pathogens, sialic acids play critical roles in mammalian cell immunity evasion and are synthesized by a conserved enzymatic pathway that includes sialic acid synthase (NeuB, SiaC, or SynC). NeuB catalyzes the condensat …
In Neisseria meningitidis and related bacterial pathogens, sialic acids play critical roles in mammalian cell immunity evasion and are synth …
Characterization of the N-acetylneuraminic acid synthase (NeuB) from the psychrophilic fish pathogen Moritella viscosa.
Berg TO, Gurung MK, Altermark B, Smalås AO, Ræder IL. Berg TO, et al. Carbohydr Res. 2015 Jan 30;402:133-45. doi: 10.1016/j.carres.2014.10.007. Epub 2014 Oct 20. Carbohydr Res. 2015. PMID: 25498013
Indeed, sialic acid can be isolated from the bacterium and when analyzed using HPLC-MS/MS, the presence of N-acetylneuraminic acid was confirmed. Thus, the N-acetylneuraminic acid synthase NeuB from M. viscosa (MvNeuB) was recombinantly produced and character …
Indeed, sialic acid can be isolated from the bacterium and when analyzed using HPLC-MS/MS, the presence of N-acetylneuraminic acid wa …
Multiple N-acetyl neuraminic acid synthetase (neuB) genes in Campylobacter jejuni: identification and characterization of the gene involved in sialylation of lipo-oligosaccharide.
Linton D, Karlyshev AV, Hitchen PG, Morris HR, Dell A, Gregson NA, Wren BW. Linton D, et al. Mol Microbiol. 2000 Mar;35(5):1120-34. doi: 10.1046/j.1365-2958.2000.01780.x. Mol Microbiol. 2000. PMID: 10712693 Free article.
N-acetyl neuraminic acid (NANA) is a common constituent of Campylobacter jejuni lipo-oligosaccharide (LOS). ...Isogenic mutants were created in all three neuB genes, and for one such mutant (neuB1) LOS was shown to have increased mobility. ...
N-acetyl neuraminic acid (NANA) is a common constituent of Campylobacter jejuni lipo-oligosaccharide (LOS). ...Isogenic mutants were …
Biosynthesis of CMP-N,N'-diacetyllegionaminic acid from UDP-N,N'-diacetylbacillosamine in Legionella pneumophila.
Glaze PA, Watson DC, Young NM, Tanner ME. Glaze PA, et al. Biochemistry. 2008 Mar 11;47(10):3272-82. doi: 10.1021/bi702364s. Epub 2008 Feb 15. Biochemistry. 2008. PMID: 18275154
In this work, the enzymes responsible for the biosynthesis and activation of N, N'-diacetyllegionaminic acid are identified for the first time. ...The NeuB homologue was found to be a N, N'-diacetyllegionaminic acid synthase that condenses 2,4-d …
In this work, the enzymes responsible for the biosynthesis and activation of N, N'-diacetyllegionaminic acid are identified fo …
Microbial production of N-acetylneuraminic acid by genetically engineered Escherichia coli.
Ishikawa M, Koizumi S. Ishikawa M, et al. Carbohydr Res. 2010 Dec 10;345(18):2605-9. doi: 10.1016/j.carres.2010.09.034. Carbohydr Res. 2010. PMID: 20971455
Previously, we described the production of N-acetylneuraminic acid (NeuAc) from N-acetylglucosamine (GlcNAc) in a system combining recombinant Escherichia coli expressing GlcNAc 2-epimerase (slr1975), E. coli expressing NeuAc synthetase (neuB), and Corynebact …
Previously, we described the production of N-acetylneuraminic acid (NeuAc) from N-acetylglucosamine (GlcNAc) in a system combi …
Characterization of N-acetylneuraminic acid synthase isoenzyme 1 from Campylobacter jejuni.
Sundaram AK, Pitts L, Muhammad K, Wu J, Betenbaugh M, Woodard RW, Vann WF. Sundaram AK, et al. Biochem J. 2004 Oct 1;383(Pt 1):83-9. doi: 10.1042/BJ20040218. Biochem J. 2004. PMID: 15200387 Free PMC article.
Escherichia coli NeuNAc (N-acetylneuraminic acid) synthase catalyses the condensation of PEP (phosphoenolpyruvate) and ManNAc (N-acetylmannosamine) to form NeuNAc and is encoded by the neuB gene. ...We determined the specificity of C. jejuni NeuNAc synthase f …
Escherichia coli NeuNAc (N-acetylneuraminic acid) synthase catalyses the condensation of PEP (phosphoenolpyruvate) and ManNAc (N …
Purification and characterization of the Escherichia coli K1 neuB gene product N-acetylneuraminic acid synthetase.
Vann WF, Tavarez JJ, Crowley J, Vimr E, Silver RP. Vann WF, et al. Glycobiology. 1997 Jul;7(5):697-701. doi: 10.1093/glycob/7.5.697. Glycobiology. 1997. PMID: 9254051
Escherichia coli K1 produces a capsular polysaccharide of alpha(2-8) poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of these neuropathogenic bacteria. ...The molecular weight of the purified enzyme is 40 kDa, similar to that predicted by …
Escherichia coli K1 produces a capsular polysaccharide of alpha(2-8) poly-N-acetylneuraminic acid. This polysaccharide is an essentia …
NeuNAc Oxime: A Slow-Binding and Effectively Irreversible Inhibitor of the Sialic Acid Synthase NeuB.
Popović V, Morrison E, Rosanally AZ, Balachandran N, Senson AW, Szabla R, Junop MS, Berti PJ. Popović V, et al. Biochemistry. 2019 Oct 15;58(41):4236-4245. doi: 10.1021/acs.biochem.9b00654. Epub 2019 Oct 4. Biochemistry. 2019. PMID: 31549502
NeuB is a bacterial sialic acid synthase used by neuroinvasive bacteria to synthesize N-acetylneuraminate (NeuNAc), helping them to evade the host immune system. NeuNAc oxime is a potent slow-binding NeuB inhibitor. It dissociated too slowly to be detected ex …
NeuB is a bacterial sialic acid synthase used by neuroinvasive bacteria to synthesize N-acetylneuraminate (NeuNAc), helping th …
Efficient Production of N-Acetylneuraminic Acid in Escherichia coli Based on the UDP-N-Acetylglucosamine Biosynthetic Pathway.
Zhao M, Zhu Y, Wang H, Zhang J, Xu W, Mu W. Zhao M, et al. J Agric Food Chem. 2023 Jul 19;71(28):10701-10709. doi: 10.1021/acs.jafc.3c02432. Epub 2023 Jul 6. J Agric Food Chem. 2023. PMID: 37409796
N-Acetylneuraminic acid (NeuAc) is the predominant sialic acid found in human cells and a human-identical milk monosaccharide. ...In this study, a NeuAc synthetic pathway was constructed in Escherichia coli BL21(DE3) by deleting the competitive pathway genes and introducin …
N-Acetylneuraminic acid (NeuAc) is the predominant sialic acid found in human cells and a human-identical milk monosaccharide. ...In …
38 results