Green synthesis of silver nanoparticles using Nardostachys jatamansi and evaluation of its anti-biofilm effect against classical colonizers

Meenakshi Sundaram Muthuraman; S Nithya; V Vinoth Kumar; Lowrence Rene Christena; Vellingiri Vadivel; Nagarajan Sai Subramanian; Savarimuthu Philip Anthony

doi:10.1016/j.micpath.2018.10.024

Green synthesis of silver nanoparticles using Nardostachys jatamansi and evaluation of its anti-biofilm effect against classical colonizers

Microb Pathog. 2019 Jan:126:1-5. doi: 10.1016/j.micpath.2018.10.024. Epub 2018 Oct 22.

Authors

Meenakshi Sundaram Muthuraman¹, S Nithya², V Vinoth Kumar³, Lowrence Rene Christena², Vellingiri Vadivel⁴, Nagarajan Sai Subramanian², Savarimuthu Philip Anthony⁵

Affiliations

¹ Department of Biotechnology, School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, 613401, India. Electronic address: msundar@biotech.sastra.edu.
² Department of Biotechnology, School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, 613401, India.
³ Department of Chemistry, School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, 613401, India.
⁴ CARISM, School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, 613401, India.
⁵ Department of Chemistry, School of Chemical & Biotechnology, SASTRA Deemed University, Thanjavur, 613401, India. Electronic address: Philip@biotech.sastra.edu.

PMID: 30352266
DOI: 10.1016/j.micpath.2018.10.024

Abstract

In this communication, we present the green synthesis of silver nanoparticles (AgNPs) using medicinally important Nardostachys jatamansi rhizome extract in the presence of sunlight. UV-vis spectroscopy, Fourier Transform Infrared spectroscopy (FTIR), Transmission electron microscope (TEM) and Energy dispersive X-ray analysis (EDX) were employed to characterize the synthesized AgNPs. UV-visible spectroscopic studies confirmed the presence of biosynthesized AgNPs. Transmission Electron Microscopic studies revealed the structure of spherical AgNPs in the diameter range of 10-15 nm. Energy dispersive X-ray analysis and elemental mapping clearly confirmed the presence of silver in AgNPs samples. Interestingly, biomolecules functionalised AgNPs exhibited a remarkable antioxidant, anti-inflammatory, and anti-biofilm activities and hence biosynthesized AgNPs from N. jatamansi can be used as a promising biomaterial for biomedical applications.

Keywords: Biomaterial; Biomedical; FTIR; Nanoparticles; Nardostachys jatamansi; TEM.

MeSH terms

Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / pharmacology
Anti-Inflammatory Agents
Antioxidants
Biofilms / drug effects*
Green Chemistry Technology / methods*
Metal Nanoparticles / chemistry*
Microbial Sensitivity Tests
Microscopy, Electron, Transmission
Nardostachys / metabolism*
Plant Extracts / chemistry
Plant Extracts / pharmacology*
Pseudomonas aeruginosa / drug effects
Silver / chemistry*
Spectrometry, X-Ray Emission
Spectroscopy, Fourier Transform Infrared
Staphylococcus aureus / drug effects

Substances

Anti-Bacterial Agents
Anti-Inflammatory Agents
Antioxidants
Plant Extracts
Silver