Upgrading volatile fatty acids production through anaerobic co-fermentation of mushroom residue and sewage sludge: Performance evaluation and kinetic analysis

Due to complex inherent structure of lignocellulosic biomass, inefficient hydrolysis and acidification limits fermentative volatile fatty acids (VFA) production of mushroom residues. Meanwhile, the mushroom residues present insufficient nutrient with a high C/N ratio. To solve this issue, anaerobic co-fermentation of cellulose-rich Oyster champost and sewage sludge was tested to enhance the VFA production, and the effect of proportion of mixed substrate was investigated in this study. The results indicated that the sewage sludge yielded higher VFAs than the Oyster champost in single-substrate fermentative system. The maximal VFA yield of 595 mgCOD/gVS_added was achieved when the proportion of sewage sludge increased to 50% in the mixed substrate. In the co-fermentation system, the optimal C/N ratio and features of mixed substrate contributed to the enhancement in hydrolysis and acidification in terms of organic solubilization and VFA production, respectively. But the co-fermentation could not increase VFA/SCOD ratio, probably due to the existence of refractory products such as humic-like and protein-like materials. Besides, this co-fermentation system had strong buffer capacity and it was not necessary to dose chemicals to control the system pH for stable VFA production. Acetate was the dominant VFA product in co-fermentation systems. A modified-Logistic model fitted co-fermentation of sludge and Oyster champost well, and presented a faster rate and higher efficiency of VFA production.