Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My NCBI Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1974 1
1976 2
1977 2
1978 6
1979 10
1980 34
1981 34
1982 43
1983 82
1984 102
1985 172
1986 207
1987 239
1988 290
1989 333
1990 399
1991 369
1992 408
1993 488
1994 491
1995 540
1996 519
1997 595
1998 551
1999 581
2000 679
2001 764
2002 782
2003 813
2004 906
2005 956
2006 988
2007 916
2008 911
2009 874
2010 914
2011 891
2012 944
2013 1005
2014 930
2015 884
2016 844
2017 803
2018 721
2019 758
2020 771
2021 969
2022 1111
2023 880
2024 349

Text availability

Article attribute

Article type

Publication date

Search Results

25,892 results

Results by year

Filters applied: . Clear all
Page 1
Showing results for mrna a. Vector
Search for Merna A. Vector instead (1 results)
Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability.
Karikó K, Muramatsu H, Welsh FA, Ludwig J, Kato H, Akira S, Weissman D. Karikó K, et al. Mol Ther. 2008 Nov;16(11):1833-40. doi: 10.1038/mt.2008.200. Epub 2008 Sep 16. Mol Ther. 2008. PMID: 18797453 Free PMC article.
The delivered mRNA and the encoded protein could be detected in the spleen at 1, 4, and 24 hours after the injection, where both products were at significantly higher levels when pseudouridine-containing mRNA was administered. Even at higher doses, only the unmodifi …
The delivered mRNA and the encoded protein could be detected in the spleen at 1, 4, and 24 hours after the injection, where both prod …
Overview of vector design for mammalian gene expression.
Kaufman RJ. Kaufman RJ. Mol Biotechnol. 2000 Oct;16(2):151-60. doi: 10.1385/MB:16:2:151. Mol Biotechnol. 2000. PMID: 11131974 Review.
The level of protein expression from heterologous genes introduced into mammalian cells depends upon multiple factors including DNA copy number, efficiency of transportation, mRNA processing, mRNA transport, mRNA stability, and translational efficiency, and p …
The level of protein expression from heterologous genes introduced into mammalian cells depends upon multiple factors including DNA copy num …
A novel retroviral vector system to analyze expression from mRNA with retained introns using fluorescent proteins and flow cytometry.
Jackson PEH, Huang J, Sharma M, Rasmussen SK, Hammarskjold ML, Rekosh D. Jackson PEH, et al. Sci Rep. 2019 Apr 23;9(1):6467. doi: 10.1038/s41598-019-42914-3. Sci Rep. 2019. PMID: 31015546 Free PMC article.
The ability to overcome cellular restrictions that exist for the export and translation of mRNAs with retained introns is a requirement for the replication of retroviruses and also for the expression of many mRNA isoforms transcribed from cellular genes. In some cases, …
The ability to overcome cellular restrictions that exist for the export and translation of mRNAs with retained introns is a requirement for …
A bicistronic vector with destabilized mRNA secondary structure yields scalable higher titer expression of human neurturin in E. coli.
Roy V, Roth R, Berge M, Chitta R, Vajrala S, Kuntumalla S, E Schmelzer A, Schoner R. Roy V, et al. Biotechnol Bioeng. 2017 Aug;114(8):1753-1761. doi: 10.1002/bit.26299. Epub 2017 May 18. Biotechnol Bioeng. 2017. PMID: 28369693
Therefore, we pursued an alternative strategy of using a bicistronic vector for NTN expression designed to reduce mRNA secondary structure to achieve increased ribosome binding and re-initiation. ...The second cistron, which contained the NTN coding sequence itself, …
Therefore, we pursued an alternative strategy of using a bicistronic vector for NTN expression designed to reduce mRNA seconda …
Optimized transfection of mRNA transcribed from a d(A/T)100 tail-containing vector.
Elango N, Elango S, Shivshankar P, Katz MS. Elango N, et al. Biochem Biophys Res Commun. 2005 May 13;330(3):958-66. doi: 10.1016/j.bbrc.2005.03.067. Biochem Biophys Res Commun. 2005. PMID: 15809089
Studies employing mRNA transfection are currently limited by a lack of transcription vectors for generating a long poly(A) tail-containing mRNA and published methods for efficient mRNA transfection. We have constructed a transcription vector con …
Studies employing mRNA transfection are currently limited by a lack of transcription vectors for generating a long poly(A) tai …
Biodistribution of onasemnogene abeparvovec DNA, mRNA and SMN protein in human tissue.
Thomsen G, Burghes AHM, Hsieh C, Do J, Chu BTT, Perry S, Barkho B, Kaufmann P, Sproule DM, Feltner DE, Chung WK, McGovern VL, Hevner RF, Conces M, Pierson CR, Scoto M, Muntoni F, Mendell JR, Foust KD. Thomsen G, et al. Nat Med. 2021 Oct;27(10):1701-1711. doi: 10.1038/s41591-021-01483-7. Epub 2021 Oct 4. Nat Med. 2021. PMID: 34608334 Clinical Trial.
One patient had improved motor function and the other died shortly after administration before appreciable clinical benefit could be observed. In both patients, onasemnogene abeparvovec DNA and messenger RNA distribution were widespread among peripheral organs and i …
One patient had improved motor function and the other died shortly after administration before appreciable clinical benefit could be observe …
Progress in Respiratory Gene Therapy.
McLachlan G, Alton EWFW, Boyd AC, Clarke NK, Davies JC, Gill DR, Griesenbach U, Hickmott JW, Hyde SC, Miah KM, Molina CJ. McLachlan G, et al. Hum Gene Ther. 2022 Sep;33(17-18):893-912. doi: 10.1089/hum.2022.172. Hum Gene Ther. 2022. PMID: 36074947 Free PMC article. Review.
Early approaches were based on gene addition, where a new DNA copy of a gene is introduced to complement a genetic mutation: however, the advent of RNA-based products that can directly express a therapeutic protein or manipulate gene expression, together with the ex …
Early approaches were based on gene addition, where a new DNA copy of a gene is introduced to complement a genetic mutation: however, …
A Novel Piggyback Strategy for mRNA Delivery Exploiting Adenovirus Entry Biology.
Lee M, Rice-Boucher PJ, Collins LT, Wagner E, Aulisa L, Hughes J, Curiel DT. Lee M, et al. Viruses. 2022 Sep 30;14(10):2169. doi: 10.3390/v14102169. Viruses. 2022. PMID: 36298724 Free PMC article.
Molecular therapies exploiting mRNA vectors embody enormous potential, as evidenced by the utility of this technology for the context of the COVID-19 pandemic. ...Our studies confirmed that AdpL could mediate effective gene transfer of mRNA vectors in …
Molecular therapies exploiting mRNA vectors embody enormous potential, as evidenced by the utility of this technology for the …
Identification of a TuMV isolate (TuMV-ZR) from Pseudostellaria heterophylla and its development into a viral expression vector.
Yang X, Gu L, Liu H, Liu C, Yuan J, Qian S, Wang J, Yuan F, Zhang Z, Mu J, Li M. Yang X, et al. Virus Res. 2023 Jul 15;332:199127. doi: 10.1016/j.virusres.2023.199127. Epub 2023 May 17. Virus Res. 2023. PMID: 37149225 Free PMC article.
A comprehensive procedure, including assembling virus-derived sRNA (vsRNA), assessing and cloning the full-length viral genome, building an infectious cloning vector and constructing a virus-based expression vector, was performed to identify viruses infecting P. het …
A comprehensive procedure, including assembling virus-derived sRNA (vsRNA), assessing and cloning the full-length viral genome, building an …
25,892 results
You have reached the last available page of results. Please see the User Guide for more information.