MRNIP is essential for meiotic progression and spermatogenesis in mice

Meng Lin; Jinxing Lv; Dan Zhao; Siyu Liu; Jinfu Xu; Yangyang Wu; Fuxin Wang; Jun Zhang; Bo Zheng; Cong Shen; Xie Guan; Jun Yu; Xiaoyan Huang

doi:10.1016/j.bbrc.2021.02.143

MRNIP is essential for meiotic progression and spermatogenesis in mice

Biochem Biophys Res Commun. 2021 Apr 23:550:127-133. doi: 10.1016/j.bbrc.2021.02.143. Epub 2021 Mar 6.

Authors

Meng Lin¹, Jinxing Lv², Dan Zhao³, Siyu Liu¹, Jinfu Xu¹, Yangyang Wu¹, Fuxin Wang⁴, Jun Zhang¹, Bo Zheng⁴, Cong Shen⁵, Xie Guan⁶, Jun Yu⁷, Xiaoyan Huang⁸

Affiliations

¹ State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.
² Suzhou Dushu Lake Hospital (Dushu Lake Hospital Affiliated to Soochow University), Suzhou, China.
³ Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, China.
⁴ Center for Reproduction and Genetics, Suzhou Municipal Hospital, Suzhou, China.
⁵ Center for Reproduction and Genetics, Suzhou Municipal Hospital, Suzhou, China. Electronic address: 344128944@qq.com.
⁶ NHC Key Laboratory of Birth Defects and Reproductive Health (Chongqing Key Laboratory of Birth Defects and Reproductive Health, Chongqing Population and Family Planning Science and Technology Research Institute), Chongqing, China. Electronic address: 38476786@qq.com.
⁷ Institute of Reproductive Medicine, Medical School, Nantong University, Nantong, China. Electronic address: yujun9117@126.com.
⁸ State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China. Electronic address: bbhxy@njmu.edu.cn.

PMID: 33689881
DOI: 10.1016/j.bbrc.2021.02.143

Abstract

Meiotic homologous recombination (HR) initiates with the programmed generation of DNA double-strand breaks (DSBs), which result in the exchange of genetic information and genome diversity. This process requires the tight cooperation of the MRE11-RAD50-NBS1 (MRN) complex to promote DSB formation and DNA end resection. However, the mechanism regulating MRN complex remains to be explored. In the present study, we report that MRN-interacting protein, MRNIP, is a novel factor for HR and is crucial for the expression of the MRN complex and loading of recombinases DMC1/RAD51. Knockout of Mrnip in mice led to aberrant synapsis, impaired HR, and male subfertility. In conclusion, MRNIP is a novel HR factor that probably promotes meiotic progression through the MRN complex.

Keywords: DNA double-strand breaks (DSBs); MRNIP; Meiotic progression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromosome Pairing* / genetics
DNA Breaks, Double-Stranded
Genes, Essential*
Homologous Recombination* / genetics
Infertility, Male / genetics
Male
Meiosis* / genetics
Mice
Mice, Knockout
Recombinational DNA Repair / genetics
Spermatogenesis* / genetics
Testis / metabolism