A peptide affinity reagent for isolating an intact and catalytically active multi-protein complex from mammalian cells.
Saathoff H, Brofelth M, Trinh A, Parker BL, Ryan DP, Low JK, Webb SR, Silva AP, Mackay JP, Shepherd NE.
Saathoff H, et al.
Bioorg Med Chem. 2015 Mar 1;23(5):960-5. doi: 10.1016/j.bmc.2015.01.023. Epub 2015 Jan 22.
Bioorg Med Chem. 2015.
PMID: 25678017
Free article.
Loading this peptide onto Streptactin beads enabled capture of the intact NuRD complex from MEL cell nuclear extract. Gentle biotin elution yielded the desired intact complex free of significant contaminants and in a form that was catalytically competent in a nucleosome re …
Loading this peptide onto Streptactin beads enabled capture of the intact NuRD complex from MEL cell nuclear extract. Gentle biotin e …