The first report on a new Tor species, Tor barakae Arunkumar & Basudha 2003, from Bangladesh using DNA barcoding technique

Md Amdadul Haque; Jonaira Rashid; Md Lipon Mia; Md Khaled Rahman; Md Azhar Ali; Anuradha Bhadra; Yahia Mahmud

doi:10.1016/j.heliyon.2023.e21764

The first report on a new Tor species, Tor barakae Arunkumar & Basudha 2003, from Bangladesh using DNA barcoding technique

Heliyon. 2023 Oct 28;9(11):e21764. doi: 10.1016/j.heliyon.2023.e21764. eCollection 2023 Nov.

Authors

Md Amdadul Haque¹, Jonaira Rashid¹, Md Lipon Mia², Md Khaled Rahman², Md Azhar Ali¹, Anuradha Bhadra¹, Yahia Mahmud³

Affiliations

¹ Bangladesh Fisheries Research Institute, Freshwater Station, Mymensingh, 2201, Bangladesh.
² Bangladesh Fisheries Research Institute, Riverine Sub-station, Rangamati, 4500, Bangladesh.
³ Bangladesh Fisheries Research Institute, Mymensingh, 2201, Bangladesh.

Abstract

Mahseer are large-scale fish of the Cyprinidae family that inhabit South and Southeast Asian mountainous streams, rivers, and reservoirs. Tor tor and Tor putitora, two species of the Tor genus, were reportedly found in Bangladesh. This study aimed to confirm the species level of specimens collected from the Sangu River. The collected samples were identified using the DNA barcoding technique, followed by amplifying 645 bp of the cytochrome oxidase c subunit 1 gene (COI) using the FishF1/FishR1 universal primer. The sequence similarity was conducted using BOLD and NCBI databases which showed 99.85-100 % similarity to the reference genome. The genetic divergence between T. putitora vs. SRI, BT, and ST was found to be 0.0239, 0.0239, and 0.0238, respectively. The genetic divergence between T. tor vs. SRI, BT, and ST was 0.0272, 0.0272, and 0.0270, respectively. In the phylogenetic tree, two clusters were formed where collected specimens (SRI, BT, and ST) formed a subcluster with the reference genome (NC_056296.1 T. barakae) with 100 % bootstrap support. This study's findings revealed the presence of a new Tor species named Tor barakae in the Sangu River basin in Bangladesh.