Promoter analysis of the bovine gene for seminalplasmin

J Herbers; K H Scheit

doi:10.1016/0167-4781(95)00139-8

Promoter analysis of the bovine gene for seminalplasmin

Biochim Biophys Acta. 1995 Sep 19;1263(3):271-4. doi: 10.1016/0167-4781(95)00139-8.

Authors

J Herbers¹, K H Scheit

Affiliation

¹ Max-Planck-Institute for Biophysical Chemistry, Department of Molecular Biology, Göttingen, Germany.

PMID: 7548218
DOI: 10.1016/0167-4781(95)00139-8

Abstract

In this study we mapped the transcriptional initiation site of the gene for seminalplasmin (SAP) by primer extension analysis, situated 125 nucleotides upstream of the translational initiation site of the SAP-specific mRNA. We showed that the TATA-box in position -30 of the SAP gene is part of a functional promoter. A 280 bp region of the 5'-flanking region exerted a strong positive effect on promoter activity. In this region we identified consensus sequences for the transcriptional control elements AP1, AP2, PEA3 and GATA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Cattle
Consensus Sequence
Molecular Sequence Data
Promoter Regions, Genetic*
Proteins / genetics*
RNA, Messenger / analysis
Seminal Vesicle Secretory Proteins*

Substances

Proteins
RNA, Messenger
Seminal Vesicle Secretory Proteins