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The 3D-structure, kinetics and dynamics of the E. coli nitroreductase NfsA with NADP+ provide glimpses of its catalytic mechanism.
FEBS Lett. 2022 Sep;596(18):2425-2440. doi: 10.1002/1873-3468.14413. Epub 2022 Jul 13.
FEBS Lett. 2022.
PMID: 35648111
Free PMC article.
The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
Day MA, Jarrom D, Christofferson AJ, Graziano AE, Anderson JLR, Searle PF, Hyde EI, White SA.
Day MA, et al.
Biochem J. 2021 Jul 16;478(13):2601-2617. doi: 10.1042/BCJ20210160.
Biochem J. 2021.
PMID: 34142705
Free PMC article.
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Oxygen-insensitive nitroreductase E. coli NfsA, but not NfsB, is inhibited by fumarate.
Day MA, Jarrom D, Rajah N, Searle PF, Hyde EI, White SA.
Day MA, et al.
Proteins. 2023 May;91(5):585-592. doi: 10.1002/prot.26451. Epub 2022 Dec 13.
Proteins. 2023.
PMID: 36443029
Free PMC article.
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Structure and Dynamics of Three Escherichia coli NfsB Nitro-Reductase Mutants Selected for Enhanced Activity with the Cancer Prodrug CB1954.
Day MA, Christofferson AJ, Anderson JLR, Vass SO, Evans A, Searle PF, White SA, Hyde EI.
Day MA, et al.
Int J Mol Sci. 2023 Mar 22;24(6):5987. doi: 10.3390/ijms24065987.
Int J Mol Sci. 2023.
PMID: 36983061
Free PMC article.
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