Microbiota decussata Kom. (Cupressaceae) is a subalpine species endemic to the Sikhote Alin Mountains with populations scattered throughout the range. We used sequence data for four noncoding regions of chloroplast DNA to characterize the genetic diversity in populations sampled from different parts of M. decussata natural range. No variation was observed in the trnT-trnF region, whereas the trnH-psbA, trnS-trnfM, and trnS-trnG regions showed polymorphisms. At the species level, we found a low nucleotide diversity (pi = 0.0009) and high haplotype diversity (h = 0.981) as well as high differentiation (Phi(ST) = 0.420). N(ST) and G(ST) values suggested the existence of a phylogeographic structure in M. decussata. The observed patterns of diversity could be explained in part by ecological features of the species and its long-term persistence throughout the range with population expansion, successive fragmentation and isolation.