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An international, interlaboratory ring trial confirms the feasibility of an extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples.
Mills MG, Bruce E, Huang ML, Crothers JW, Hyrien O, Oura CAL, Blake L, Brown Jordan A, Hester S, Wehmas L, Mari B, Barby P, Lacoux C, Fassy J, Vial P, Vial C, Martinez JRW, Oladipo OO, Inuwa B, Shittu I, Meseko CA, Chammas R, Santos CF, Dionísio TJ, Garbieri TF, Parisi VA, Mendes-Correa MC, de Paula AV, Romano CM, Góes LGB, Minoprio P, Campos AC, Cunha MP, Vilela APP, Nyirenda T, Mkakosya RS, Muula AS, Dumm RE, Harris RM, Mitchell CA, Pettit S, Botten J, Jerome KR. Mills MG, et al. Among authors: cunha mp. PLoS One. 2022 Jan 13;17(1):e0261853. doi: 10.1371/journal.pone.0261853. eCollection 2022. PLoS One. 2022. PMID: 35025926 Free PMC article.
Yellow Fever Virus RNA in Urine and Semen of Convalescent Patient, Brazil.
Barbosa CM, Di Paola N, Cunha MP, Rodrigues-Jesus MJ, Araujo DB, Silveira VB, Leal FB, Mesquita FS, Botosso VF, Zanotto PMA, Durigon EL, Silva MV, Oliveira DBL. Barbosa CM, et al. Among authors: cunha mp. Emerg Infect Dis. 2018 Jan;24(1):176-8. doi: 10.3201/eid2401.171310. Epub 2018 Jan 17. Emerg Infect Dis. 2018. PMID: 29058663 Free PMC article.
Prolonged Shedding of Zika Virus Associated with Congenital Infection.
Oliveira DB, Almeida FJ, Durigon EL, Mendes ÉA, Braconi CT, Marchetti I, Andreata-Santos R, Cunha MP, Alves RP, Pereira LR, Melo SR, Neto DF, Mesquita FS, Araujo DB, Favoretto SR, Sáfadi MA, Ferreira LC, Zanotto PM, Botosso VF, Berezin EN. Oliveira DB, et al. Among authors: cunha mp. N Engl J Med. 2016 Sep 22;375(12):1202-4. doi: 10.1056/NEJMc1607583. Epub 2016 Aug 24. N Engl J Med. 2016. PMID: 27653589 Free article. No abstract available.
An international, interlaboratory ring trial confirms the feasibility of an open-source, extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples.
Mills MG, Bruce E, Huang ML, Crothers JW, Hyrien O, Oura CAL, Blake L, Jordan AB, Hester S, Wehmas L, Mari B, Barby P, Lacoux C, Fassy J, Vial P, Vial C, Martinez JRW, Oladipo OO, Inuwa B, Shittu I, Meseko CA, Chammas R, Santos CF, José Dionísio T, Garbieri TF, Parisi VA, Mendes-Correa MC, dePaula AV, Romano CM, Góes LGB, Minoprio P, Campos AC, Cunha MP, Vilela APP, Nyirenda T, Mkakosya RS, Muula AS, Dumm RE, Harris RM, Mitchell CA, Pettit S, Botten J, Jerome KR. Mills MG, et al. Among authors: cunha mp. medRxiv [Preprint]. 2021 Apr 16:2021.04.10.21254091. doi: 10.1101/2021.04.10.21254091. medRxiv. 2021. PMID: 33880478 Free PMC article. Updated. Preprint.
Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System.
Oliveira DBL, Durigon GS, Mendes ÉA, Ladner JT, Andreata-Santos R, Araujo DB, Botosso VF, Paola ND, Neto DFL, Cunha MP, Braconi CT, Alves RPS, Jesus MR, Pereira LR, Melo SR, Mesquita FS, Silveira VB, Thomazelli LM, Favoretto SR, Almonfrey FB, Abdulkader RCRM, Gabrili JM, Tambourgi DV, Oliveira SF, Prieto K, Wiley MR, Ferreira LCS, Silva MV, Palacios GF, Zanotto PMA, Durigon EL. Oliveira DBL, et al. Among authors: cunha mp. Viruses. 2018 Nov 7;10(11):615. doi: 10.3390/v10110615. Viruses. 2018. PMID: 30405055 Free PMC article.