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M. botterel
(17 results)?
One-step warming does not affect the in vitro viability and cryosurvival of cryotop-vitrified donkey embryos.
Theriogenology. 2020 Aug;152:47-52. doi: 10.1016/j.theriogenology.2020.04.026. Epub 2020 Apr 22.
Theriogenology. 2020.
PMID: 32361488
Embryos were then randomly warmed using two different warming procedures: (i) W3 (three-step dilution; n = 11): embryos were warmed in 1 M, 0.5 M and 0 M sucrose, and (ii) W1/0.5 (one-step dilution; n = 9): embryos were warmed directly in 0.5 M sucrose …
Embryos were then randomly warmed using two different warming procedures: (i) W3 (three-step dilution; n = 11): embryos were warmed in 1 …
Stallion sperm freezing with sucrose extenders: A strategy to avoid permeable cryoprotectants.
Consuegra C, Crespo F, Bottrel M, Ortiz I, Dorado J, Diaz-Jimenez M, Pereira B, Hidalgo M.
Consuegra C, et al.
Anim Reprod Sci. 2018 Apr;191:85-91. doi: 10.1016/j.anireprosci.2018.02.013. Epub 2018 Feb 21.
Anim Reprod Sci. 2018.
PMID: 29482878
In Experiment 1, extenders containing 1% of BSA and different concentrations of sucrose (mmol/L, M): 0, 50, 100, 250, 350 and 450 mM were compared. Use of sucrose [100 mM (S2)] resulted in greater values for most of the sperm kinetic parameters assessed (P < 0.001). ...
In Experiment 1, extenders containing 1% of BSA and different concentrations of sucrose (mmol/L, M): 0, 50, 100, 250, 350 and 450 mM …
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The cryoprotective effect of Ficoll 70 on the post-warming survival and quality of Cryotop-vitrified donkey embryos.
Bottrel M, Mogas T, Pereira B, Ortiz I, Díaz-Jiménez M, Consuegra C, Hidalgo M, Morató R, Dorado J.
Bottrel M, et al.
Theriogenology. 2020 May;148:180-185. doi: 10.1016/j.theriogenology.2019.11.013. Epub 2019 Nov 13.
Theriogenology. 2020.
PMID: 31757484
Before vitrification, embryos were randomly distributed into two groups: (i) VS1 (n = 14): vitrified using 15% EG + 15% DMSO + 0.5 M sucrose; and (ii) VS2 (n = 10): vitrified in the same medium supplemented also with 18% of Ficoll 70. ...
Before vitrification, embryos were randomly distributed into two groups: (i) VS1 (n = 14): vitrified using 15% EG + 15% DMSO + 0.5 M …
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Vitrification in straws conserves motility features better than spheres in donkey sperm.
Diaz-Jimenez M, Dorado J, Pereira B, Ortiz I, Consuegra C, Bottrel M, Ortiz E, Hidalgo M.
Diaz-Jimenez M, et al.
Reprod Domest Anim. 2018 Sep;53 Suppl 2:56-58. doi: 10.1111/rda.13256.
Reprod Domest Anim. 2018.
PMID: 30238658
Ejaculates from six Andalusian donkeys were collected and extended in Gent without glycerol supplemented with sucrose 0.1 M (Molar). Samples were slowly cooled at 5C. For vitrification, 30 mul suspensions (spheres) were dropped directly into liquid nitrogen (LN(2) ) or fil …
Ejaculates from six Andalusian donkeys were collected and extended in Gent without glycerol supplemented with sucrose 0.1 M (Molar). …
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