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Showing results for M. al shawi
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Inhibition of F1-ATPase rotational catalysis by the carboxyl-terminal domain of the ϵ subunit.
Nakanishi-Matsui M, Sekiya M, Yano S, Futai M. Nakanishi-Matsui M, et al. J Biol Chem. 2014 Oct 31;289(44):30822-30831. doi: 10.1074/jbc.M114.578872. Epub 2014 Sep 16. J Biol Chem. 2014. PMID: 25228697 Free PMC article.
The subunit, an endogenous inhibitor, lowers F1-ATPase activity by decreasing the rotation speed and extending the duration of the inhibited state (Sekiya, M., Hosokawa, H., Nakanishi-Matsui, M., Al-Shawi, M. K., Nakamoto, R. K., and Futai, M …
The subunit, an endogenous inhibitor, lowers F1-ATPase activity by decreasing the rotation speed and extending the duration of the inhibited …
The global impact of adverse childhood experiences on criminal behavior: A cross-continental study.
Basto-Pereira M, Gouveia-Pereira M, Pereira CR, Barrett EL, Lawler S, Newton N, Stapinski L, Prior K, Costa MSA, Ximenes JM, Rocha AS, Michel G, Garcia M, Rouchy E, Al Shawi A, Sarhan Y, Fulano C, Magaia AJ, El-Astal S, Alattar K, Sabbah K, Holtzhausen L, Campbell E, Villanueva L, Gomis-Pomares A, Adrián JE, Cuervo K, Sakulku J. Basto-Pereira M, et al. Child Abuse Negl. 2022 Feb;124:105459. doi: 10.1016/j.chiabu.2021.105459. Epub 2022 Jan 8. Child Abuse Negl. 2022. PMID: 35007971
PARTICIPANTS AND SETTING: In total, 3797 young adults aged between 18 and 20 years (M = 18.97; DP = 0.81) were assessed locally in community settings within the 10 countries. ...
PARTICIPANTS AND SETTING: In total, 3797 young adults aged between 18 and 20 years (M = 18.97; DP = 0.81) were assessed locally in co …
A rotor-stator cross-link in the F1-ATPase blocks the rate-limiting step of rotational catalysis.
Scanlon JA, Al-Shawi MK, Nakamoto RK. Scanlon JA, et al. J Biol Chem. 2008 Sep 19;283(38):26228-40. doi: 10.1074/jbc.M804858200. Epub 2008 Jul 15. J Biol Chem. 2008. PMID: 18628203 Free PMC article.
To understand the gamma subunit role in the catalytic mechanism, we previously determined the partial rate constants and devised a minimal kinetic model for the rotational hydrolytic mode of the F(1)-ATPase enzyme that uniquely fits the pre-steady state and steady state data ( Ba …
To understand the gamma subunit role in the catalytic mechanism, we previously determined the partial rate constants and devised a minimal k …
Transition state analysis of the coupling of drug transport to ATP hydrolysis by P-glycoprotein.
Al-Shawi MK, Polar MK, Omote H, Figler RA. Al-Shawi MK, et al. J Biol Chem. 2003 Dec 26;278(52):52629-40. doi: 10.1074/jbc.M308175200. Epub 2003 Oct 9. J Biol Chem. 2003. PMID: 14551217 Free article.
We used purified human Pgp (ABCB1, MDR1) expressed in Saccharomyces cerevisiae (Figler, R. A., Omote, H., Nakamoto, R. K., and Al-Shawi, M. K. (2000) Arch. Biochem. Biophys. 376, 34-46) as well as Chinese hamster Pgp (PGP1). ...These results are consistent wi …
We used purified human Pgp (ABCB1, MDR1) expressed in Saccharomyces cerevisiae (Figler, R. A., Omote, H., Nakamoto, R. K., and Al- …
Single molecule behavior of inhibited and active states of Escherichia coli ATP synthase F1 rotation.
Sekiya M, Hosokawa H, Nakanishi-Matsui M, Al-Shawi MK, Nakamoto RK, Futai M. Sekiya M, et al. J Biol Chem. 2010 Dec 31;285(53):42058-67. doi: 10.1074/jbc.M110.176701. Epub 2010 Oct 25. J Biol Chem. 2010. PMID: 20974856 Free PMC article.
ATP hydrolysis-dependent rotation of the F(1) sector of the ATP synthase is a successive cycle of catalytic dwells (0.2 ms at 24 C) and 120 rotation steps (0.6 ms) when observed under V(max) conditions using a low viscous drag 60-nm bead attached to the gamma subunit (Sekiya, …
ATP hydrolysis-dependent rotation of the F(1) sector of the ATP synthase is a successive cycle of catalytic dwells (0.2 ms at 24 C) and 120 …
Allosteric interactions between the two non-equivalent nucleotide binding domains of multidrug resistance protein MRP1.
Hou Y, Cui L, Riordan JR, Chang X. Hou Y, et al. J Biol Chem. 2000 Jul 7;275(27):20280-7. doi: 10.1074/jbc.M001109200. J Biol Chem. 2000. PMID: 10781583 Free article.
In the well studied P-glycoprotein multidrug transporter, the two appear to be functionally equivalent, and a strongly supported model proposes that ATP hydrolysis occurs alternately at each NBD (Senior, A. E., al-Shawi, M. K., and Urbatsch, I. L. (1995) FEBS …
In the well studied P-glycoprotein multidrug transporter, the two appear to be functionally equivalent, and a strongly supported model propo …
Determination of the partial reactions of rotational catalysis in F1-ATPase.
Scanlon JA, Al-Shawi MK, Le NP, Nakamoto RK. Scanlon JA, et al. Biochemistry. 2007 Jul 31;46(30):8785-97. doi: 10.1021/bi700610m. Epub 2007 Jul 10. Biochemistry. 2007. PMID: 17620014
On the basis of the testing of all possible minimal kinetic models, this step must follow hydrolysis and precede Pi release in order to explain burst kinetics. Consistent with the single molecule analysis of Yasuda et al. (Yasuda, R., Noji, H., Yoshida, M., Kinosita …
On the basis of the testing of all possible minimal kinetic models, this step must follow hydrolysis and precede Pi release in order to expl …
Characterization of the ATPase activity of purified Chinese hamster P-glycoprotein.
Urbatsch IL, al-Shawi MK, Senior AE. Urbatsch IL, et al. Biochemistry. 1994 Jun 14;33(23):7069-76. doi: 10.1021/bi00189a008. Biochemistry. 1994. PMID: 7911680
A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, …
A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell lin …
Synthesis and hydrolysis of ATP and the phosphate-ATP exchange reaction in soluble mitochondrial F1 in the presence of dimethylsulfoxide.
Tuena de Gómez-Puyou M, Pérez-Hernández G, Gómez-Puyou A. Tuena de Gómez-Puyou M, et al. Eur J Biochem. 1999 Dec;266(2):691-6. doi: 10.1046/j.1432-1327.1999.00915.x. Eur J Biochem. 1999. PMID: 10561614 Free article.
In medium containing 40% dimethylsulfoxide, soluble F1 catalyzes the hydrolysis of ATP introduced at concentrations lower than that of the enzyme [Al-Shawi, M.K. & Senior, A.E. (1992), Biochemistry 31, 886-891]. At this concentration of dimethylsulfoxide, …
In medium containing 40% dimethylsulfoxide, soluble F1 catalyzes the hydrolysis of ATP introduced at concentrations lower than that of the e …
Effects of lipids on ATPase activity of purified Chinese hamster P-glycoprotein.
Urbatsch IL, Senior AE. Urbatsch IL, et al. Arch Biochem Biophys. 1995 Jan 10;316(1):135-40. doi: 10.1006/abbi.1995.1020. Arch Biochem Biophys. 1995. PMID: 7840607 Free article.
Chinese hamster P-glycoprotein ("multidrug-resistance protein") was purified and reconstituted in proteoliposomes by the procedure of I. L. Urbatsch, M. K. al-Shawi, and A. E. Senior (1994, Biochemistry 33, 7069-7076). ...
Chinese hamster P-glycoprotein ("multidrug-resistance protein") was purified and reconstituted in proteoliposomes by the procedure of I. L. …
18 results