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Page 1
Cellular Toxicity Mechanisms and the Role of Autophagy in Pt(IV) Prodrug-Loaded Ultrasmall Iron Oxide Nanoparticles Used for Enhanced Drug Delivery.
Gutiérrez-Romero L, Rivas-García L, Sánchez-González C, Llopis J, Blanco E, Montes-Bayón M. Gutiérrez-Romero L, et al. Pharmaceutics. 2021 Oct 19;13(10):1730. doi: 10.3390/pharmaceutics13101730. Pharmaceutics. 2021. PMID: 34684023 Free PMC article.
Quantitative Pt results revealed that after 24 h exposure to 20 M Pt in the form of the Pt(IV)-loaded nanoparticles, approximately 10% of the incorporated Pt was associated with nuclear DNA. ...
Quantitative Pt results revealed that after 24 h exposure to 20 M Pt in the form of the Pt(IV)-loaded nanoparticles, approximately 10 …
Study of phytochelatins and other related thiols as complexing biomolecules of As and Cd in wild type and genetically modified Brassica juncea plants.
Navaza AP, Montes-Bayón M, LeDuc DL, Terry N, Sanz-Medel A. Navaza AP, et al. J Mass Spectrom. 2006 Mar;41(3):323-31. doi: 10.1002/jms.992. J Mass Spectrom. 2006. PMID: 16421878
In both the Cd and As exposed plants it was possible to observe the presence of oxidized PC2 ([M + H]+, m/z 538), GS-PC2(-Glu) ([M + H]+, m/z 716) as well as reduced GSH ([M + H]+, m/z 308) and oxidized glutathione (GSSG) ([M + H]+ …
In both the Cd and As exposed plants it was possible to observe the presence of oxidized PC2 ([M + H]+, m/z 538), GS-PC2(-Glu) …
Overexpressing both ATP sulfurylase and selenocysteine methyltransferase enhances selenium phytoremediation traits in Indian mustard.
LeDuc DL, AbdelSamie M, Móntes-Bayon M, Wu CP, Reisinger SJ, Terry N. LeDuc DL, et al. Environ Pollut. 2006 Nov;144(1):70-6. doi: 10.1016/j.envpol.2006.01.008. Epub 2006 Mar 2. Environ Pollut. 2006. PMID: 16515825
To this end we incorporated a gene (encoding selenocysteine methyltransferase, SMT) from the Se hyperaccumulator, Astragalus bisulcatus, into Indian mustard (LeDuc, D.L., Tarun, A.S., Montes-Bayon, M., Meija, J., Malit, M.F., Wu, C.P., AbdelSamie, M …
To this end we incorporated a gene (encoding selenocysteine methyltransferase, SMT) from the Se hyperaccumulator, Astragalus bisulcatus, int …
Sample preparation strategies for quantitative analysis of catalase in red blood cells by elemental mass spectrometry.
Mudarra Rubio A, Montes-Bayón M, Blanco-González E, Sanz-Medel A. Mudarra Rubio A, et al. Metallomics. 2010 Sep;2(9):638-45. doi: 10.1039/c0mt00003e. Epub 2010 Aug 13. Metallomics. 2010. PMID: 21072355
Further fractionation of Fe-containing proteins from the preconcentrated extract was achieved by size exclusion chromatography (Superdex 200) with a mobile phase of ammonium acetate (0.05 M, pH 7.4) coupled to ICP-MS (Fe monitoring) and UV/VIS detection (specific absorptio …
Further fractionation of Fe-containing proteins from the preconcentrated extract was achieved by size exclusion chromatography (Superdex 200 …
Anion exchange chromatography for the determination of 5-methyl-2'-deoxycytidine: application to cisplatin-sensitive and cisplatin-resistant ovarian cancer cell lines.
Iglesias T, Espina M, Montes-Bayón M, Sierra LM, Blanco-González E. Iglesias T, et al. Anal Bioanal Chem. 2015 Mar;407(9):2423-31. doi: 10.1007/s00216-014-8070-7. Epub 2014 Aug 21. Anal Bioanal Chem. 2015. PMID: 25142048
Good separation of the five target nucleotides, including 5-methyl-2'-deoxycytidine monophosphate (5mdCMP) and 2'-deoxycytidine monophosphate (dCMP) was achieved on the Mono-Q column with a gradient elution of ammonium acetate buffer (1 M, pH 6.9) at a flow rate of 1 mL mi …
Good separation of the five target nucleotides, including 5-methyl-2'-deoxycytidine monophosphate (5mdCMP) and 2'-deoxycytidine monophosphat …
Titanium release in serum of patients with different bone fixation implants and its interaction with serum biomolecules at physiological levels.
Nuevo-Ordóñez Y, Montes-Bayón M, Blanco-González E, Paz-Aparicio J, Raimundez JD, Tejerina JM, Peña MA, Sanz-Medel A. Nuevo-Ordóñez Y, et al. Anal Bioanal Chem. 2011 Nov;401(9):2747-54. doi: 10.1007/s00216-011-5232-8. Epub 2011 Jul 23. Anal Bioanal Chem. 2011. PMID: 21785984
Minimizing sample handling and therefore contamination issues, we obtained detection limits of about 0.05 mug L(-1) Ti working at medium resolution (m/deltam 4000). Such extremely good sensitivity permitted us to establish the range of Ti concentration in serum of 40 contr …
Minimizing sample handling and therefore contamination issues, we obtained detection limits of about 0.05 mug L(-1) Ti working at medium res …
New strategy to address DNA-methyl transferase activity in ovarian cancer cell cultures by monitoring the formation of 5-methylcytosine using HPLC-UV.
Iglesias González T, Blanco-González E, Montes-Bayón M. Iglesias González T, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Aug 15;1028:16-24. doi: 10.1016/j.jchromb.2016.05.038. Epub 2016 May 26. J Chromatogr B Analyt Technol Biomed Life Sci. 2016. PMID: 27318640
For this aim, a correlation between the enzyme activity and the concentration of 5mC obtained by HPLC-UV is previously obtained under optimized conditions using both, un-methylated and hemi-methylated DNA substrates and the prokaryotic methyltransferase M.SssI as model enz …
For this aim, a correlation between the enzyme activity and the concentration of 5mC obtained by HPLC-UV is previously obtained under optimi …
Strategies to study human serum transferrin isoforms using integrated liquid chromatography ICPMS, MALDI-TOF, and ESI-Q-TOF detection: application to chronic alcohol abuse.
del Castillo Busto ME, Montes-Bayón M, Blanco-González E, Meija J, Sanz-Medel A. del Castillo Busto ME, et al. Anal Chem. 2005 Sep 1;77(17):5615-21. doi: 10.1021/ac050574s. Anal Chem. 2005. PMID: 16131073
MALDI-TOF appeared to be sensitive to concentration levels of the analytes, and the observed mass accuracy was highly compromised by the protein heterogeneity (peak width at half-maximum approximately 2000 Da for every fraction). On the other hand, ESI-Q-TOF allowed good mass acc …
MALDI-TOF appeared to be sensitive to concentration levels of the analytes, and the observed mass accuracy was highly compromised by the pro …
Selenium species in aqueous extracts of alfalfa sprouts by two-dimensional liquid chromatography coupled to inductively coupled plasma mass spectrometry and electrospray mass spectrometry detection.
Gergely V, Montes-Bayón M, Fodor P, Sanz-Medel A. Gergely V, et al. J Agric Food Chem. 2006 Jun 28;54(13):4524-30. doi: 10.1021/jf060320n. J Agric Food Chem. 2006. PMID: 16786993
Finally, an additional Se-containing species showing Se isotope distribution was detected at a molecular ion m/z 239 in the ESI-Q-TOF. The collision-induced dissociation of the m/z 239 and 237 ions (corresponding to 80Se and 78Se isotopes, respectively) revealed the …
Finally, an additional Se-containing species showing Se isotope distribution was detected at a molecular ion m/z 239 in the ESI-Q-TOF …
Determination of total homocysteine in human serum by capillary gas chromatography with sulfur-specific detection by double focusing ICP-MS.
de la Flor St Rèmy RR, Montes-Bayón M, Sanz-Medel A. de la Flor St Rèmy RR, et al. Anal Bioanal Chem. 2003 Sep;377(2):299-305. doi: 10.1007/s00216-003-2075-y. Epub 2003 Jul 3. Anal Bioanal Chem. 2003. PMID: 12844208
Detection was carried out by means of a double-focusing inductively coupled plasma mass spectrometer (DF-ICP-MS) monitoring (32)S at m/Delta m (resolving power)=3000. The transfer line used to transport the analytes from the GC column to the ICP-MS had previously be …
Detection was carried out by means of a double-focusing inductively coupled plasma mass spectrometer (DF-ICP-MS) monitoring (32)S at m …