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Year Number of Results
1992 1
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2002 1
2003 1
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2006 2
2007 1
2009 2
2011 5
2012 5
2013 2
2015 2
2018 1
2019 1
2020 1
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2022 1
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29 results

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Page 1
First Dye-Decolorizing Peroxidase from an Ascomycetous Fungus Secreted by Xylaria grammica.
Kimani V, Ullrich R, Büttner E, Herzog R, Kellner H, Jehmlich N, Hofrichter M, Liers C. Kimani V, et al. Biomolecules. 2021 Sep 21;11(9):1391. doi: 10.3390/biom11091391. Biomolecules. 2021. PMID: 34572604 Free PMC article.
RESULTS: The enzyme oxidizes classic peroxidase substrates such as 2,6-DMP but also veratryl alcohol and notably Mn(2+) to Mn(3+) ions, suggesting a physiological function of this DyP in lignin modification. The K(M) value (49 M) indicates that Mn(2+) ions bind with …
RESULTS: The enzyme oxidizes classic peroxidase substrates such as 2,6-DMP but also veratryl alcohol and notably Mn(2+) to Mn(3+) ions, sugg …
Molecular dynamics simulations of heme reorientational motions in myoglobin.
Henry ER. Henry ER. Biophys J. 1993 Mar;64(3):869-85. doi: 10.1016/S0006-3495(93)81447-4. Biophys J. 1993. PMID: 8471731 Free PMC article.
However, an estimate of the order parameter for rapid internal motions of the heme from those orientations which are sampled by the simulations suggests that the subnanosecond orientational dynamics of the heme accounts for at least 30% of the unresolved initial anisotropy decay …
However, an estimate of the order parameter for rapid internal motions of the heme from those orientations which are sampled by the simulati …
A spectrophotometric assay for the detection of fungal peroxygenases.
Poraj-Kobielska M, Kinne M, Ullrich R, Scheibner K, Hofrichter M. Poraj-Kobielska M, et al. Anal Biochem. 2012 Feb 1;421(1):327-9. doi: 10.1016/j.ab.2011.10.009. Epub 2011 Oct 8. Anal Biochem. 2012. PMID: 22037293
The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425 nm (epsilon(425)=9,700 M(-1) cm(-1)), and a red color when adjusted to pH >12, which can be measured in form of an end-point determination at 514 nm (epsilon(514)=11,40 …
The product, 4-nitrocatechol, produces a yellow color at pH 7, which can be followed over time at 425 nm (epsilon(425)=9,700 M(-1) cm …
Heme-thiolate ferryl of aromatic peroxygenase is basic and reactive.
Wang X, Ullrich R, Hofrichter M, Groves JT. Wang X, et al. Proc Natl Acad Sci U S A. 2015 Mar 24;112(12):3686-91. doi: 10.1073/pnas.1503340112. Epub 2015 Mar 10. Proc Natl Acad Sci U S A. 2015. PMID: 25759437 Free PMC article.
Kinetic characterization showed that APO-II is unusually reactive toward a panel of benzylic C-H and phenolic substrates, with second-order rate constants for C-H and O-H bond scission in the range of 10-10(7) M(-1)s(-1). Our results demonstrate the important role of the a …
Kinetic characterization showed that APO-II is unusually reactive toward a panel of benzylic C-H and phenolic substrates, with second-order …
Molecular fungal community and its decomposition activity in sapwood and heartwood of 13 temperate European tree species.
Leonhardt S, Hoppe B, Stengel E, Noll L, Moll J, Bässler C, Dahl A, Buscot F, Hofrichter M, Kellner H. Leonhardt S, et al. PLoS One. 2019 Feb 14;14(2):e0212120. doi: 10.1371/journal.pone.0212120. eCollection 2019. PLoS One. 2019. PMID: 30763365 Free PMC article.
In this study, we intensively analyzed the molecular fungal community composition and species richness in relation to extracellular enzyme activity and differences in decomposing sapwood and heartwood of 13 temperate tree species (four coniferous and nine deciduous species, log d …
In this study, we intensively analyzed the molecular fungal community composition and species richness in relation to extracellular enzyme a …
Steroid hydroxylation by basidiomycete peroxygenases: a combined experimental and computational study.
Babot ED, Del Río JC, Cañellas M, Sancho F, Lucas F, Guallar V, Kalum L, Lund H, Gröbe G, Scheibner K, Ullrich R, Hofrichter M, Martínez AT, Gutiérrez A. Babot ED, et al. Appl Environ Microbiol. 2015 Jun 15;81(12):4130-42. doi: 10.1128/AEM.00660-15. Epub 2015 Apr 10. Appl Environ Microbiol. 2015. PMID: 25862224 Free PMC article.
Interestingly, antiviral and other biological activities of 25-hydroxycholesterol have been reported recently (M. Blanc et al., Immunity 38:106-118, 2013, http://dx.doi.org/10.1016/j.immuni.2012.11.004). ...
Interestingly, antiviral and other biological activities of 25-hydroxycholesterol have been reported recently (M. Blanc et al., Immun …
Oxidative cleavage of diverse ethers by an extracellular fungal peroxygenase.
Kinne M, Poraj-Kobielska M, Ralph SA, Ullrich R, Hofrichter M, Hammel KE. Kinne M, et al. J Biol Chem. 2009 Oct 23;284(43):29343-9. doi: 10.1074/jbc.M109.040857. Epub 2009 Aug 27. J Biol Chem. 2009. PMID: 19713216 Free PMC article.
Investigations with several model substrates provided information about the route for ether cleavage: (a) steady-state kinetics results with methyl 3,4-dimethoxybenzyl ether, which was oxidized to 3,4-dimethoxybenzaldehyde, gave parallel double reciprocal plots suggestive of a pi …
Investigations with several model substrates provided information about the route for ether cleavage: (a) steady-state kinetics results with …
Epoxidation of linear, branched and cyclic alkenes catalyzed by unspecific peroxygenase.
Peter S, Kinne M, Ullrich R, Kayser G, Hofrichter M. Peter S, et al. Enzyme Microb Technol. 2013 May 10;52(6-7):370-6. doi: 10.1016/j.enzmictec.2013.02.013. Epub 2013 Mar 6. Enzyme Microb Technol. 2013. PMID: 23608506
The apparent Michaelis-Menten constant (Km) for the epoxidation of the model substrate 2-methyl-2-butene was 5mM, the turnover number (kcat) 1.310(3)s(-1) and the calculated catalytic efficiency, kcat/Km, was 2.510(5)M(-1)s(-1). As epoxides represent chemical building bloc …
The apparent Michaelis-Menten constant (Km) for the epoxidation of the model substrate 2-methyl-2-butene was 5mM, the turnover number (kcat) …
29 results