Feasibility of pressurization to speed up enzymatic hydrolysis of biological materials for multielement determinations.
Moreda-Piñeiro A, Bermejo-Barrera A, Bermejo-Barrera P, Moreda-Piñeiro J, Alonso-Rodríguez E, Muniategui-Lorenzo S, López-Mahía P, Prada-Rodríguez D.
Moreda-Piñeiro A, et al.
Anal Chem. 2007 Mar 1;79(5):1797-805. doi: 10.1021/ac061379w. Epub 2007 Feb 2.
Anal Chem. 2007.
PMID: 17269790
For pepsin, optimized conditions (pH 1.0, temperature 40 degrees C, pressure 1500 psi, static time 2 min, and number of cycles 3) gave quantitative extractions for As, Cd, Co, Cu, Hg, Li, Mn, Pb, Se, Sr, V, and Zn. ...For pancreatin, quantitative recoveries were only reach …
For pepsin, optimized conditions (pH 1.0, temperature 40 degrees C, pressure 1500 psi, static time 2 min, and number of cycles 3) gav …